Spectral heterogeneity and carotenoid-to-bacteriochlorophyll energy transfer in LH2 light-harvesting complexes from Allochromatium vinosum

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• 作者：Nikki M. Magdaong ; Amy M. LaFountain ; Kirsty Hacking…
• 关键词：Carotenoids ; Energy transfer ; Light ; harvesting ; Photosynthesis ; Pigment ; protein complex ; Ultrafast transient absorption spectroscopy
• 刊名：Photosynthesis Research
• 出版年：2016
• 出版时间：February 2016
• 年：2016
• 卷：127
• 期：2
• 页码：171-187
• 全文大小：2,304 KB
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• 作者单位：Nikki M. Magdaong (1)
  Amy M. LaFountain (1)
  Kirsty Hacking (2)
  Dariusz M. Niedzwiedzki (3)
  George N. Gibson (4)
  Richard J. Cogdell (2)
  Harry A. Frank (1)
  
  1. Department of Chemistry, University of Connecticut, 55 North Eagleville Road, Storrs, CT, 06269-3060, USA
  2. Institute of Molecular Cell and Systems Biology, University of Glasgow, Glasgow, Scotland, UK
  3. Photosynthetic Antenna Research Center, Washington University in St. Louis, St. Louis, MO, USA
  4. Department of Physics, University of Connecticut, 2152 Hillside Road, Storrs, CT, 06269-3046, USA
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Plant Physiology
  
• 出版者：Springer Netherlands
• ISSN：1573-5079

文摘

Photosynthetic organisms produce a vast array of spectral forms of antenna pigment-protein complexes to harvest solar energy and also to adapt to growth under the variable environmental conditions of light intensity, temperature, and nutrient availability. This behavior is exemplified by Allochromatium (Alc.) vinosum, a photosynthetic purple sulfur bacterium that produces different types of LH2 light-harvesting complexes in response to variations in growth conditions. In the present work, three different spectral forms of LH2 from Alc. vinosum, B800-820, B800-840, and B800-850, were isolated, purified, and examined using steady-state absorption and fluorescence spectroscopy, and ultrafast time-resolved absorption spectroscopy. The pigment composition of the LH2 complexes was analyzed by high-performance liquid chromatography, and all were found to contain five carotenoids: lycopene, anhydrorhodovibrin, spirilloxanthin, rhodopin, and rhodovibrin. Spectral reconstructions of the absorption and fluorescence excitation spectra based on the pigment composition revealed significantly more spectral heterogeneity in these systems compared to LH2 complexes isolated from other species of purple bacteria. The data also revealed the individual carotenoid-to-bacteriochlorophyll energy transfer efficiencies which were correlated with the kinetic data from the ultrafast transient absorption spectroscopic experiments. This series of LH2 complexes allows a systematic exploration of the factors that determine the spectral properties of the bound pigments and control the rate and efficiency of carotenoid-to-bacteriochlorophyll energy transfer. Keywords Carotenoids Energy transfer Light-harvesting Photosynthesis Pigment-protein complex Ultrafast transient absorption spectroscopy