RNA-binding protein RBM8A (Y14) and MAGOH localize to centrosome in human A549 cells

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• 作者：Yasuhito Ishigaki (1)
  Yuka Nakamura (1)
  Takanori Tatsuno (1)
  Mitsumasa Hashimoto (2) (3)
  Kuniyoshi Iwabuchi (3)
  Naohisa Tomosugi (1) (4)
  
• 关键词：RBM8A (Y14) ; MAGOH ; Cell cycle ; Centrosome
• 刊名：Histochemistry and Cell Biology
• 出版年：2014
• 出版时间：January 2014
• 年：2014
• 卷：141
• 期：1
• 页码：101-109
• 全文大小：1,102 KB
• 作者单位：Yasuhito Ishigaki (1)
  Yuka Nakamura (1)
  Takanori Tatsuno (1)
  Mitsumasa Hashimoto (2) (3)
  Kuniyoshi Iwabuchi (3)
  Naohisa Tomosugi (1) (4)
  
  1. Medical Research Institute, Kanazawa Medical University, 1-1 Daigaku, Uchinada-machi, Kahoku, 920-0293, Japan
  2. Department of Physics, Kanazawa Medical University, 1-1 Daigaku, Uchinada-machi, Kahoku, 920-0293, Japan
  3. Department of Biochemistry, Kanazawa Medical University, 1-1 Daigaku, Uchinada-machi, Kahoku, 920-0293, Japan
  4. Medical Care Proteomics Biotechnology Co., Ltd., 1-1 Daigaku, Uchinada-machi, Kahoku, 920-0293, Japan
  
• ISSN：1432-119X

文摘

RBM8A (Y14) is carrying RNA-binding motif and forms the tight heterodimer with MAGOH. The heterodimer is known to be a member of exon junction complex on exporting mRNA and is required for mRNA metabolisms such as splicing, mRNA export and nonsense-mediated mRNA decay. Almost all RBM8A–MAGOH complexes localize in nucleoplasm and shuttle between nuclei and cytoplasm for RNA metabolism. Recently, the abnormality of G2/M transition and aberrant centrosome regulation in RBM8A- or MAGOH-deficient cells has been reported. These results prompt us to the reevaluation of the localization of RBM8A–MAGOH in human cells. Interestingly, our immunostaining experiments showed the localization of these proteins in centrosome in addition to nuclei. Furthermore, the transiently expressed eYFP-tagged RBM8A and Flag-tagged MAGOH also co-localized with centrosome signals. In addition, the proximity ligation in situ assay was performed to detect the complex formation in centrosome. Our experiments clearly showed that Myc-tagged RBM8A and Flag-tagged MAGOH formed a complex in centrosome. GFP-tagged PLK1 also co-localized with Myc-RBM8A. Our results show that RBM8A–MAGOH complex is required for M-phase progression via direct localization to centrosome rather than indirect effect.