A phylogenetic network of wild Ussurian pears (Pyrus ussuriensis Maxim.) in China revealed by hypervariable regions of chloroplast DNA

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• 作者：Tana Wuyun (1)
  Teng Ma (1)
  Chiyomi Uematsu (2)
  Hironori Katayama (3)
  
• 关键词：Wild Ussurian pear ; Pyrus ussuriensis ; Genetic diversity ; Chloroplast DNA ; Conservation
• 刊名：Tree Genetics & Genomes
• 出版年：2013
• 出版时间：February 2013
• 年：2013
• 卷：9
• 期：1
• 页码：167-177
• 全文大小：541KB
• 参考文献：1. Bandelt HJ, Forster P, Roehl A (1999) Median-joining networks for inferring intraspecific phylogenies. Mol Biol Evol 16:27-8 CrossRef
  2. Bao L, Chen K, Zhang D, Li X, Teng Y (2008) An assessment of genetic variability and relationships within Asian pears based on AFLP (amplified fragment length polymorphism) markers. Sci Hort 116:374-80 CrossRef
  3. Cao Y, Tian L, Gao Y, Liu F (2012) Genetic diversity of cultivated and Ussurian pear ( / Pyrus ussuriensis Maxim.) in China evaluated with M13-tailed SSR markers. Genet Resour Crop Evol 59:9-7 CrossRef
  4. Coart E, Vekemans X, Smulders MM, Wagner I, Van Huylenbroeck J, Van Bockstaele E, Roldan-Ruiz I (2003) Genetic variation in the endangered wild apple ( / Malus sylvestris (L.) Mill.) in Belgium as revealed by amplified fragment length polymorphism and microsatellite markers. Mol Ecol 12:845-57 CrossRef
  5. Coart E, Van Glabeke S, De Loose M, Larsen AS, Roldan-Ruzi I (2006) Chloroplast diversity in the genus / Malus: new insights into the relationship between the European wild apple ( / Malus sylvestris (L.) Mill.) and the domesticated apple ( / Malus domestica Borkh.). Mol Ecol 15:2171-182 CrossRef
  6. Heng W, Zhang S, Fang C, Wu H, Wu J (2008) Identification of 20S-genotypes and cloning novel S-RNases in / Pyrus. Acta Horticulturae Sinica 35:313-18 (in Chinese with English summary)
  7. Iketani H, Ohashi H (2003) Taxonomy and distribution of Japanese populations of / Pyrus ussuriensis Maxim. (Rosaceae). J Jpn Bot 78:119-34
  8. Iketani H, Yamamoto T, Katayama H, Uematsu C, Mase N, Sato Y (2010) Introgression between native and prehistorically naturalized (archaeophytic) wild pear ( / Pyrus spp.) population in Northern Tohoku, Northern Japan. Conserv Genet 11:115-26 CrossRef
  9. Jiao PJ, Guo TJ, Zhang FY, Li JH, Lu WP, Liu ZG, Guo ZG (2000) Research on the evaluation of main characters of the / Pyrus ussuriensis Maxim. SpecWild Econ Anim Plant Res 1:24-8, in Chinese
  10. Kajiura I (1983) Nihon nashi no kigen to chiriteki bunka. In: Akihama T (ed) Ikushugaku Saikin no Shinpo, Youkendo, Tokyo, vol. 25:3-3 (in Japanese)
  11. Katayama H, Uematsu C (2003) Comparative analysis of chloroplast DNA in / Pyrus species: physical map and gene localization. Theor Appl Genet 106:303-10
  12. Katayama H, Uematsu C (2006) Pear ( / Pyrus species) genetic resources in Iwate, Japan. Genet Resour Crop Evol 53:483-98 CrossRef
  13. Katayama H, Adachi S, Yamamoto T, Uematsu C (2007) A wide range of genetic diversity in pear ( / Pyrus ussuriensis var. / aromatica) genetic resources from Iwate, Japan revealed by SSR and chloroplast DNA markers. Genet Resour Crop Evol 54:1573-585 CrossRef
  14. Katayama H, Tachibana M, Iketani H, Zhang SL, Uematsu C (2012) Phylogenetic utility of structural alterations found in the chloroplast genome of pear: hypervariable regions in a highly conserved genome. Tree Genet Genomes 8:313-26
  15. Kikuchi A (1948) Nashi. Kajuengeigaku Jyo-kan, Kajyusyurui kakuron, Yokendo, Tokyo, pp. 64-16 (in Japanese)
  16. Liu X, Liu D (1995) Study on the resources development of / Pyrus ussuriensis Maxim. from Yichun forest region. Quarterly of forest by-product and speciality in China 1 (in Chinese)
  17. Ma?T, Wuyun T, Deng W, Kang X, Luo J (2011) Investigation on wild / Pyrus ussuriensis Maxim. resources of Chifeng area in Inner Mongolia. Guangzhou Agricultural Science .2 (in Chinese with English summary)
  18. Ministry of the Environment, the Government of Japan (2007) The updated japanese red lists on mammals, brackish-water/freshwater fishes, insects, shellfish, and plants I and II (in Japanese)
  19. Ohwi J (1965) Flora of Japan. Smithsonian Institution, Washington, p 549
  20. Report of the Chinese Academy of Agricultural Science (1963) China fruit records, Shanghai Technology House (in Chinese with English summary)
  21. Roberson A, Newton AC, Ennos RA (2004) Multiple hybrid origins genetic diversity and population genetic structure of two endemic / Sorbus taxa on the Isle of Arran, Scotland. Mol Ecol 13:123-34 CrossRef
  22. Sun Y, Li BJ, Zhang MJ (2008) Studies about the characteristics of flower and pollen of / Pyrus ussuriensis. Northern fruits. 3 (in Chinese with English summary)
  23. Teng Y, Tanabe K, Tamura F, Itai A (2002) Genetic relationships of / Pyrus species and cultivars native to East Asia revealed by randomly amplified polymorphic DNA markers. J Am Soc Hort Sci 127:262-70
  24. Terakami S, Matsumura Y, Kurita K, Kanamori H, Katayose Y, Yamamoto T, Katayama H (2012) Complete sequence of the chloroplast genome from pear ( / Pyrus pyrifolia): genome structure and comparative analysis. Tree Genet Genomes. doi:10.1007/s11295-012-0469-8
  25. Yu TT (1979) Taxonomy of the fruit tree in China. Agriculture Press, Beijing (in Chinese)
  26. Zhang M, Li B, Wang Q, Ding L, Yan X, Xing G (2007) Study on cross-compatibility of cultivars and wild types of / Pyrus ussuriensis. J Fruit Sci 24:427-32 (in Chinese with English summary)
  27. Zhang M, Wang Q, Li B, Ding L, Yan X, Xing G (2009) Identification of S-genotypes on the pear cultivars and wild forms of / Pyrus ussuriensis. J Fruit Sci 26:786-91 (in Chinese with English summary)
  28. Zhang MJ, Wang Q, Ding LH, Yan XK, Chui YZ (2010) Studies on pollen characters and fertility of cultivars and wild types of / Pyrus ussuriensis. J Jilin Agric Sci 35:53-7 (in Chinese with English summary)
  
• 作者单位：Tana Wuyun (1)
  Teng Ma (1)
  Chiyomi Uematsu (2)
  Hironori Katayama (3)
  
  1. Key Laboratory of Cultivation and Protection for Non-Wood Forest Trees and Non-wood Forest Products of SFA, Central South University of Forestry and Technology, Changsha, Hunan, 410004, People’s Republic of China
  2. Botanical Gardens, Graduate School of Science, Osaka City University, Osaka, 576-0004, Japan
  3. Food Resources Education and Research Center, Kobe University, Hyogo, 675-2103, Japan
  

文摘

In order to understand the genetic diversity of wild Ussurian pears in China, chloroplast DNA (cpDNA) of 186 wild accessions from 12 populations in Inner Mongolia, Heilongjiang and Jilin Provinces and 51 Chinese and European pear cultivars including Pyrus ussuriensis, Pyrus pyrifolia, Pyrus bretschneideri, Pyrus sinkiangensis and Pyrus communis were investigated. Each accession was classified into one of three types (types A, B and C) based on two large deletions in the hypervariable regions between the accD–psaI and rps16–trnQ genes. Thirty haplotypes were identified by 32 mutations including 17 gaps (in/dels) and 15 base changes. Haplotype network analysis revealed that wild Chinese Ussurian pears could be grouped into subgroup I of type A. A haplotype, Hcp3, in subgroup I detected in Heilongjiang and Jilin Provinces was considered to be a divergent centre in Chinese Ussurian pears. However, the genetic diversity of wild accessions revealed by the two hypervariable regions was quite low. In particular, 98?% of wild Ussurian accessions in Inner Mongolia shared an identical haplotype Hcp1 and are, therefore, monomorphic. In comparison, Chinese pear cultivars were more divergent. These results suggest that the cpDNAs from wild Ussurian pears in Inner Mongolia have specifically differentiated compared to those from pears of other areas. The number of wild Ussurian pears has been decreasing because of desertification and land development, therefore conservation is needed.