Rice false smut pathogen, Ustilaginoidea virens, invades through small gap at the apex of a rice spikelet before heading

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• 作者：Taketo Ashizawa (1) toketa@affrc.go.jp
  Mami Takahashi (1)
  Michiyoshi Arai (1)
  Tsutomu Arie (2)
• 关键词：Rice – ; Spikelet – ; Villosiclava – ; Green fluorescent protein – ; Conidia
• 刊名：Journal of General Plant Pathology
• 出版年：2012
• 出版时间：July 2012
• 年：2012
• 卷：78
• 期：4
• 页码：255-259
• 全文大小：658.7 KB
• 参考文献：1. Ashizawa T, Kataoka Y (2005) Detection of Ustilaginoidea virens in rice panicles before and after heading in the field using nested-PCR technique with species-specific primers (in Japanese with English summary). Jpn J Phytopathol 71:16–19
  2. Ashizawa T, Takahashi M, Moriwaki J, Hirayae K (2010) Quantification of the rice false smut pathogen Ustilaginoidea virens from soil in Japan using real-time PCR. Eur J Plant Pathol 128:221–232
  3. Ashizawa T, Takahashi M, Moriwaki J, Hirayae K (2011) A refined inoculation method to evaluate false smut resistance in rice. J Gen Plant Pathol 77:10–16
  4. Fujita Y, Sonoda R, Yaegashi H (1989) Inoculation with conidiospores of false smut fungus to rice panicles at the booting stage (in Japanese with English summary). Ann Phytopath Soc Japan 55:629–634
  5. Ikegami H (1961) Studies on the false smut of rice VII. Analysis on the occurrence of the smut balls at the different ages (in Japanese with English summary). Res Bull Fac Agric Gifu Univ 14:27–37
  6. Ikegami H (1963) Studies on the false smut of rice X. Invasion of chlamydospores and hyphae of the false smut fungus into rice plants. Res Bull Fac Agric Gifu Univ 18:54–60
  7. Kepler RM, Sung G-H, Harada Y, Tanaka K, Tanaka E, Hosoya T, Bischoff JF, Spatafora JW (2012) Host jumping onto close relatives and across kingdoms by Tyrannicordyceps (Clavicipitaceae) gen. nov. and Ustilaginoidea (Clavicipitaceae). Am J Bot 99:552–561
  8. Nakamura K, Izumiyama N, Ohtsubo K, Koiso Y, Iwasaki S, Sonoda R, Fujita Y, Yaegashi H, Sato Z (1992) Lupinosis in mice caused by ustiloxin and a crude extract of fungal culture of Ustilaginoidea virens (in Japanese with English summary). Proc Jpn Assoc Mycotoxicol 35:41–43
  9. Prasher DC, Eckenrode VK, Ward WW, Prendergast FG, Cormier MJ (1992) Primary structure of the Aequorea victoria green-fluorescent protein. Gene 111:229–233
  10. Satake T (1972) Circular dense-culture of rice plants in pots, the purpose of obtaining many uniform panicles of main stems (in Japanese). Jpn J Crop Sci 41:361–362
  11. Shimomura O, Johnson FH, Saiga Y (1962) Extraction, purification and properties of aequorin, a bioluminescent protein from the luminous hydromedusan, Aequorea. J Cell Comp Physiol 59:223–239
  12. Sonoda R, Fujita Y, Yaegashi H (1988) Distribution of the number of the false smut balls produced on rice panicle (in Japanese). Ann Rept Plant Prot North Jpn 39:92–93
  13. Suwa M (1915) Study on Ina-kouji Part 1 (in Japanese). JAMAS (Igaku Chuo Zasshi, Japan) 13:661–689
  14. Tanaka E, Ashizawa T, Sonoda R, Tanaka C (2008) Villosiclava virens gen. nov., com. nov., teleomorph of Ustilaginoidea virens, the causal agent of rice false smut. Mycotaxon 106:491–501
  15. Tanaka E, Kumagawa T, Tanaka C, Koga H (2011) Simple transformation of the rice false smut fungus Villosiclava virens by electroporation of intact conidia. Mycosci 52:344–348
  16. Watanabe S, Kumakura K, Izawa N, Nagayama K, Mitachi T, Kanamori M, Teraoka T, Arie T (2007) Mode of action of Trichoderma asperellum SKT-1, a biocontrol agent against Gibberella fujikuroi. J Pestic Sci 32:222–228
  17. White JF Jr, Sullivan R, Moy M, Patel R, Duncan R (2000) An overview of problems in the classification of plant-parasitic Clavicipitaceae. Stud Mycol 45:95–105
  18. Zhang Z, Du X, Chai R, Mao X, Qui H, Wang Y, Wang J, Sun G (2006) Agrobacterium tumefaciens-mediated transformation of the pathogen of Ustilaginoidea virens (in Chinese). Chin J Rice Sci 20:440–442
• 作者单位：1. Hokuriku Paddy Cropping System Project Team, Hokuriku Research Center, National Agricultural Research Center, National Agriculture and Food Research Organization, 1-2-1 Inada, Joetsu, Niigata 943-0193, Japan2. Laboratory of Plant Pathology, Tokyo University of Agriculture and Technology, 3-5-8 Saiwaicho, Fuchu, Tokyo 183-8509, Japan
• ISSN：1610-739X

文摘

Ustilaginoidea virens, the false smut pathogen of rice, produces false smut balls on spikelets after heading. To clarify how the fungus invades spikelets during the booting stage, we developed a fungal strain that expresses a green fluorescent protein gene and injected conidia from this strain into rice sheaths. Observations at 48 h post-inoculation showed many conidia were present on spikelet surfaces, and the conidia had germinated and the hyphae have gradually grown by 120 h post-inoculation. By 144 h, hyphae had invaded spikelets through their apices, via the small gap between the lemma and palea and had already reached all floral organs.