Pineapple translation factor SUI1 and ribosomal protein L36 promoters drive constitutive transgene expression patterns in Arabidopsis thaliana
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  • 作者:Jonni Koia (1)
    Richard Moyle (1)
    Caroline Hendry (1)
    Lionel Lim (1)
    José Ramón Botella (1)
  • 关键词:Ribosomal protein L36 ; SUI1 ; Promoter ; Constitutive ; GUS ; Pineapple ; Arabidopsis
  • 刊名:Plant Molecular Biology
  • 出版年:2013
  • 出版时间:5 - March 2013
  • 年:2013
  • 卷:81
  • 期:4
  • 页码:327-336
  • 全文大小:668KB
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  • 作者单位:Jonni Koia (1)
    Richard Moyle (1)
    Caroline Hendry (1)
    Lionel Lim (1)
    José Ramón Botella (1)

    1. University of Queensland, Brisbane, 4072, Australia
  • ISSN:1573-5028
文摘
The availability of a variety of promoter sequences is necessary for the genetic engineering of plants, in basic research studies and for the development of transgenic crops. In this study, the promoter and 5-untranslated regions of the evolutionally conserved protein translation factor SUI1 gene and ribosomal protein L36 gene were isolated from pineapple and sequenced. Each promoter was translationally fused to the GUS reporter gene and transformed into the heterologous plant system Arabidopsis thaliana. Both the pineapple SUI1 and L36 promoters drove GUS expression in all tissues of Arabidopsis at levels comparable to the CaMV35S promoter. Transient assays determined that the pineapple SUI1 promoter also drove GUS expression in a variety of climacteric and non-climacteric fruit species. Thus the pineapple SUI1 and L36 promoters demonstrate the potential for using translation factor and ribosomal protein genes as a source of promoter sequences that can drive constitutive transgene expression patterns.

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