FDR and SDR processes in meiosis and diploid gamete formation in poplars (Populus L.) detected by centromere-associated microsatellite markers
详细信息 查看全文
- 作者：Heike Liesebach (1)
Kristina Ulrich (2)
Dietrich Ewald (2) - 关键词：Populus sp. ; Triploid ; Unreduced gametes ; Microsatellite markers ; Heterozygosity ; Poplar breeding
- 刊名：Tree Genetics & Genomes
- 出版年：2015
- 出版时间：February 2015
- 年：2015
- 卷：11
- 期：1
- 全文大小：450 KB
- 参考文献：1. Bradshaw HD, Stettler RF (1993) Molecular genetics of growth and development in / Populus. I. Triploidy in hybrid poplars. Theor Appl Genet 86:301-07. doi:10.1007/bf00222092
2. Brownfield L, K?hler C (2011) Unreduced gamete formation in plants: mechanisms and prospects. J Exp Bot 62:1659-668. doi:10.1093/jxb/erq371 CrossRef
3. Cai X, Xu SS (2007) Meiosis-driven genome variation in plants. Curr Genom 8:151-61 CrossRef
4. Cuenca J, Froelicher Y, Aleza P, Juarez J, Navarro L, Ollitrault P (2011) Multilocus half-tetrad analysis and centromere mapping in citrus: evidence of SDR mechanism for 2n megagametophyte production and partial chiasma interference in mandarin cv ‘Fortune- Heredity 107:462-70. doi:10.1038/hdy.2011.33 CrossRef
5. De Storme N, Geelen D (2013) Sexual polyploidization in plants—cytological mechanisms and molecular regulation. New Phytol 198:670-84. doi:10.1111/nph.12184 CrossRef
6. De Storme N, Copenhaver GP, Geelen D (2012) Production of diploid male gametes in / Arabidopsis by cold-induced destabilization of postmeiotic radial microtubule arrays. Plant Physiol 160:1808-826. doi:10.1104/pp. 112.208611 CrossRef
7. Dewitte A, Van Laere K, Van Huylenbroeck J (2012) Use of 2n gametes in plant breeding. In: Abdurakhmonov IY (ed) Plant breeding. Agricultural and Biological Sciences. pp 59-6. doi: 10.5772/29827
8. Dong C-B, Suo Y-J, Kang X-Y (2014) Assessment of the genetic composition of triploid hybrid / Populus using SSR markers with low recombination frequencies. Can J Forest Res:692-99 doi: 10.1139/cjfr-2013-0360
9. Dumolin S, Demesure B, Petit RJ (1995) Inheritance of chloroplast and mitochondrial genomes in pedunculate oak investigated with an efficient PCR method. Theor Appl Genet 91:1253-256. doi:10.1007/BF00220937 CrossRef
10. Esselink GD, Nybom H, Vosman B (2004) Assignment of allelic configuration in polyploids using the MAC-PR (microsatellite DNA allele counting—peak ratios) method. Theor Appl Genet 109:402-08. doi:10.1007/s00122-004-1645-5 CrossRef
11. Ewald D, Ulrich K (2012) In vitro pollination in poplar of section / Populus. Plant Cell Tiss Organ Cult 111:255-58. doi:10.1007/s11240-012-0189-7 CrossRef
12. Ewald D, Ulrich K, Naujoks G, Schr?der MB (2009) Induction of tetraploid poplar and black locust plants using colchicine: chloroplast number as an early marker for selecting polyploids in vitro. Plant Cell Tiss Organ Cult 99:353-57. doi:10.1007/s11240-009-9601-3 CrossRef
13. Ewald D, Ulrich K, Liesebach H (2012) Erzeugung triploider Individuen und intersektioneller Hybriden bei verschiedenen Pappelarten. In: Züchtung und Ertragsleistung schnellwachsender Baumarten im Kurzumtrieb - Erkenntnisse aus drei Jahren FastWOOD, ProLoc und Weidenzüchtung, Hann. Münden. Beitr?ge aus der Nordwestdeutschen Forstlichen Versuchsanstalt, pp 183-93
14. Ferrante S, Lucretti S, Reale S, Patrizio A, Abbate L, Tusa N, Scarano M-T (2010) Assessment of the origin of new citrus tetraploid hybrids (2n =4x) by means of SSR markers and PCR based dosage effects. Euphytica 173:223-33. doi:10.1007/s10681-009-0093-3 CrossRef
15. Jackson N, Sanchez-Moran E, Buckling E, Armstrong SJ, Jones GH, Franklin FCH (2006) Reduced meiotic crossovers and delayed prophase I progression in AtMLH3-deficient / Arabidopsis. EMBO J 25:1315-323. doi:10.1038/sj.emboj.76 - 作者单位：Heike Liesebach (1)
Kristina Ulrich (2)
Dietrich Ewald (2)
1. Thünen Institute of Forest Genetics, German Federal Research Institute for Rural Areas, Forestry and Fisheries, Sieker Landstrasse 2, 22927, Grosshansdorf, Germany
2. Thünen Institute of Forest Genetics, German Federal Research Institute for Rural Areas, Forestry and Fisheries, Eberswalder Chaussee 3A, 15377, Waldsieversdorf, Germany - ISSN：1614-2950
文摘
Sexual polyploidisation is one of the appropriate approaches in poplar breeding. Controlled pollinations were carried out with spontaneously formed, as well as induced, 2n gametes. Among the offspring individuals, 36 triploid plants and 1 tetraploid individual were detected by flow cytometry. The parental clones and all polyploid offspring individuals were genotyped by 18 nuclear microsatellite markers. The allelic configurations, especially tri-allelic patterns, and dosage effects were used to recognise diploid contributions of the male or female gamete. Three out of 18 markers localised near the centromeres of linkage groups I, X and XV. They are assumed to be unaffected by crossing over events and, therefore, able to ascertain the mechanism of first division restitution (FDR) or second division restitution (SDR) to generate diploid gametes. The applied three unlinked centromere-associated microsatellite markers allow a very effective determination of FDR resp. SDR processes. Altogether, 21 diploid pollen (10 FDR and 11 SDR) and 13 diploid ovules (1 FDR and 12 SDR) as well as 2 cases of postmeiotic reconstitution were determined with no inconsistency for the three markers. A female hybrid aspen clone (Populus tremula?×-em class="a-plus-plus">Populus tremuloides) was assured to be able to frequently spontaneously form diploid ovules by the SDR mechanism. The transferred average heterozygosity in FDR gametes was assessed to be remarkably higher than that in SDR gametes. However, a selective inducement to favour FDR gametes seems not to be feasible with the current thermo-treatment techniques.