Heat Shock Protein 27 Expression is Inversely Correlated with Atrophic Gastritis and Intraepithelial Neoplasia

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• 作者：Yoshiaki Nagata (1)
  Masatoshi Kudo (1)
  Tomoyuki Nagai (1)
  Tomohiro Watanabe (2)
  Masanori Kawasaki (1)
  Yutaka Asakuma (1)
  Satoru Hagiwara (1)
  Naoshi Nishida (1)
  Shigenaga Matsui (1)
  Hiroshi Kashida (1)
  Toshiharu Sakurai (1)
  
• 关键词：Gastric cancer ; HSP27 ; ROS ; Inflammation ; Atrophic gastritis ; EMT ; Stem cell
• 刊名：Digestive Diseases and Sciences
• 出版年：2013
• 出版时间：February 2013
• 年：2013
• 卷：58
• 期：2
• 页码：381-388
• 全文大小：472KB
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• 作者单位：Yoshiaki Nagata (1)
  Masatoshi Kudo (1)
  Tomoyuki Nagai (1)
  Tomohiro Watanabe (2)
  Masanori Kawasaki (1)
  Yutaka Asakuma (1)
  Satoru Hagiwara (1)
  Naoshi Nishida (1)
  Shigenaga Matsui (1)
  Hiroshi Kashida (1)
  Toshiharu Sakurai (1)
  
  1. Department of Gastroenterology and Hepatology, Faculty of Medicine, Kinki University, 377-2, Ohno-Higashi, Osaka-Sayama, Osaka, 589-8511, Japan
  2. Center for Innovation in Immunoregulative Technology and Therapeutics, Kyoto University Graduate School of Medicine, 54 Kawahara-cho, Shogoin, Sakyo-ku, Kyoto, 606-8507, Japan
  
• ISSN：1573-2568

文摘

Background Intestinal-type gastric carcinomas progress through several sequential steps, including atrophic gastritis, intestinal metaplasia, dysplasia, and cancer. Aim We investigated heat shock protein 27 (HSP27) expression in gastric neoplasia and background gastric mucosa to assess its involvement in gastric carcinogenesis. Methods We used real-time quantitative polymerase chain reaction to examine HSP27 expression in gastric neoplasias and background gastric mucosae of 30 patients with intraepithelial neoplasias and in gastric mucosae of 30 patients without gastric neoplasia. Immunohistochemical staining was performed on 30 advanced gastric cancer tissues. Results HSP27 expression was negatively associated with atrophic gastritis. HSP27 expression in the background gastric mucosa of neoplasia-bearing patients was significantly lower than in the mucosa of those without gastric neoplasia. In tumor necrosis factor α-treated gastric cancer cells, HSP27 knockdown increased cell death and accumulation of the reactive oxygen species that link inflammation to cancer. Poorly differentiated tumors most frequently had high HSP27 levels. Dedifferentiation of cancer cells is associated with an epithelial–mesenchymal transition (EMT) signaling pathway. In gastric cancer MKN-1 cells, HSP27 knockdown upregulated E-cadherin and downregulated vimentin and smooth muscle actin, but this did not occur in MKN-74 cells. Conclusion HSP27 expression in gastric mucosae is inversely correlated with intraepithelial neoplasia, a probable precursor to gastric cancer, and HSP27 expression in cancer is positively correlated with poor differentiation.