Analysis of Promoter Region of X-Linked pgk-1 Gene Polymorphisms: Evidence for Polyclonality of Adult Mouse Gastric Glands
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- 作者：Sachiyo Nomura ; Michio Kaminishi ; Nobuo Takagi and Hiroyasu Esumi
- 关键词：clonality ; stomach ; gastric gland ; X ; chromosome inactivation ; pgk ; 1 ; DNA methylation
- 刊名：Digestive Diseases and Sciences
- 出版年：2004
- 出版时间：February, 2004
- 年：2004
- 卷：49
- 期：2
- 页码：218-223
- 全文大小：6.1 KB
文摘
The clonality of gastric glands remains a controversial topic. Chimeric mouse studies suggested that all gastric glands were monoclonal. However, using the X-linked transgenic mouse model, we have suggested that most glands are polyclonal during development and that the fraction of monoclonal glands increases after birth. Nevertheless, a fraction of glands is perpetually polyclonal even in the adult murine stomach. To examine the existence of gastric polyclonal glands in the adult mouse, we studied an X-linked intrinsic polymorphic gene, pgk-1, in 6 week-old female mice heterozygous for the X-linked pgk-1a and pgk-1b. The sequence containing the seventh HpaII site in the promoter region of the gene and the polymorphic sites was utilized. Twenty-four of 225 fundic glands (10.7 % ) and 3 of 167 pyloric glands (1.8 % ) were clonally heterotypic. Only 0.6 % of colonic crypts were heterotypic. Clonally heterotypic glands with inactive X-specific methylation were present in the adult murine stomach.