In Vitro and Numerical Support for Combinatorial Irreversible Electroporation and Electrochemotherapy Glioma Treatment

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• 作者：R. E. Neal II (1) (2)
  J. H. Rossmeisl Jr. (3)
  V. D’Alfonso (3)
  J. L. Robertson (4)
  P. A. Garcia (1)
  S. Elankumaran (5)
  R. V. Davalos (1)
  
• 关键词：Combined therapy ; Non ; thermal focal ablation ; Brain cancer ; IRE ; ECT ; Numerical modeling ; Minimally invasive surgery ; Multimodality oncology ; Targeted therapy
• 刊名：Annals of Biomedical Engineering
• 出版年：2014
• 出版时间：March 2014
• 年：2014
• 卷：42
• 期：3
• 页码：475-487
• 全文大小：1,515 KB
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• 作者单位：R. E. Neal II (1) (2)
  J. H. Rossmeisl Jr. (3)
  V. D’Alfonso (3)
  J. L. Robertson (4)
  P. A. Garcia (1)
  S. Elankumaran (5)
  R. V. Davalos (1)
  
  1. Bioelectromechanical Systems Lab, Virginia Tech -Wake Forest School of Biomedical Engineering and Sciences, Blacksburg, VA, USA
  2. Radiology Research Unit, The Alfred Hospital, 55 Commercial Road, Melbourne, VIC, 3004, Australia
  3. Neurology/Neurosurgery Service and Center for Comparative Oncology, VA-MD Regional College of Veterinary Medicine, Blacksburg, VA, USA
  4. Cancer Engineering Group, Virginia Tech -Wake Forest School of Biomedical Engineering and Sciences, Blacksburg, VA, USA
  5. Department of Biomedical Sciences and Pathobiology, VA-MD Regional College of Veterinary Medicine, Blacksburg, VA, USA
  
• ISSN：1573-9686

文摘

Irreversible electroporation (IRE) achieves targeted volume non-thermal focal ablation using a series of brief electric pulses to kill cells by disrupting membrane integrity. Electrochemotherapy (ECT) uses lower numbers of sub-lethal electric pulses to disrupt membranes for improved drug uptake. Malignant glioma (MG) brain tumors are difficult to treat due to diffuse peripheral margins into healthy neural tissue. Here, in vitro experimental data and numerical simulations investigate the feasibility for IRE-relevant pulse protocols with adjuvant ECT drugs to enhance MG treatment. Cytotoxicity curves were produced on two glioma cell lines in vitro at multiple pulse strengths and drug doses with Bleomycin or Carboplatin. Pulses alone increased cytotoxicity with higher pulse numbers and strengths, reaching >90% by 800?V/cm with 90 pulses. Chemotherapeutic addition increased cytotoxicity by >50% for 1?ng/mL concentrations of either drug relative to 80 pulses alone with J3T cells at electric fields ?00?V/cm. In addition to necrosis, transmission electron microscopy visualizes apoptotic morphological changes and Hoescht 33342 staining shows apoptotic cell fractions varying with electric field and drug dose relative to controls. Numerically simulated treatment volumes in a canine brain show IRE combined with ECT expands therapeutic volume by 2.1-.2 times compared to IRE alone.