Genoprevalence in human tissues of TT-virus genotype 6

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• 作者：L. Kakkola ; N. Kaipio ; K. Hokynar ; P. Puolakkainen ; P. S. Mattila ; A. Kokkola ; E. K. Partio ; A.-M. Eis-H¨¹binger ; M. S?derlund-Venermo and K. Hedman
• 刊名：Archives of Virology
• 出版年：2004
• 出版时间：June 2004
• 年：2004
• 卷：149
• 期：6
• 页码：1095-1106
• 全文大小：113 KB

文摘

TT virus (TTV) is a newly discovered human virus of high genotypic diversity. TTV is widely distributed among humans, but the possible genotype-related differences in TTV biology are not well known. The prevalence and amount of TTV-DNA, especially of genotype 6, was determined by nested-PCR in various human tissues, and human parvovirus B19, another ssDNA virus, was used as a reference. TTV DNA was detected simultaneously in bile, peripheral blood mononuclear cells (PBMC) and plasma of 77 % subjects, in 38 % skin samples, in 38 % synovial samples and in all (100 % ) adenoids, tonsils and liver samples. The relative concentrations of TTV-DNA did not vary significantly among the different samples. Genotype 6 TTV-DNA was detected in bile and plasma of one subject (3 % ), in skin and serum of one subject (8 % ) and in one liver (5 % ). The overall prevalence of TTV genotype 6 was 4 % in subjects and 4 % in sera. TTV genotype 6 was shown to occur in human tissues with no obvious tissue-type or symptom specificity. Parvovirus B19 DNA was detected overall in 38 % subjects, and bile was the only sample type tested that did not persistently harbour B19 DNA.