Evaluation of differential protein expression in Haliclona aquarius and sponge-associated microorganisms under cadmium stress

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• 作者：Rodrigo Cunha Wanick ; Herbert de Sousa Barbosa…
• 关键词：Sponge proteomics ; Ecotoxicoproteomics ; Cadmium ; 2 ; D gel electrophoresis ; Mass spectrometry ; Stress response ; Trace metals
• 刊名：Analytical and Bioanalytical Chemistry
• 出版年：2013
• 出版时间：September 2013
• 年：2013
• 卷：405
• 期：24
• 页码：7661-7670
• 全文大小：441KB
• 参考文献：1. Hansen IV, Weeks JM, Depledge MH (1995) Mar Pollut Bull 31:133-38 CrossRef
  2. Schr?der HC, Hassanein HM, Lauenroth S, Koziol C, Mohamed TA, Lacorn M, Steinhart H, Batel R, Müller WE (1999) Arch Environ Contam Toxicol 36:47-5 CrossRef
  3. Waisberg MP, Joseph B, Beyersmann HD (2003) Toxicology 192:95-17 CrossRef
  4. Huang X, Fang C-W, Guo Y-W, Huang H-Q (2011) Ecotox Environ Saf 74:1232-237 CrossRef
  5. Martín-Díaz ML, Blasco J, Sales D, DelValls TA (2004) Trends Anal Chem 23:10-1
  6. Lafi FF, Garson MJ, Fuerst JA (2005) Microb Ecol 50:213-20 CrossRef
  7. Lee KY, Lee J, Lee HK (2001) J Microbiol 39:254-64
  8. Taylor MW, Radax R, Steger D, Wagner M (2007) Microbiol Mol Biol R 71:295-47 CrossRef
  9. Viarengo A, Ponzano E, Dondero F, Fabbri RAA (1997) Mar Environ Res 44:69-4 CrossRef
  10. Bradford MM (1976) Anal Biochem 72:248-54 CrossRef
  11. Berkelman T, Stenstedt T (1998) 2-D electrophoresis—principles and methods. Amersham Biosciences, Uppsala
  12. Ling XP, Zhua J-Y, Huanga L, Huang H-Q (2009) Environ Toxicol Phar 27:212-18 CrossRef
  13. Zhou Q, Zhang J, Fu J, Shi J, Jiang G (2008) Anal Chim Acta 606:135-50 CrossRef
  14. Amiard J-C, Amiard-Triquet C, Barka S, Pellerin J, Rainbow PS (2006) Aquat Toxicol 76:160-02 CrossRef
  15. Erk M, Ivankovic D, Raspor B, Pavicic J (2002) Talanta 57:1211-218 CrossRef
  16. Geret F, Rainglet F, Cosson RP (1998) Mar Environ Res 46:545-50 CrossRef
  17. Muralidharan S, Thompson E, Raftos D, Birch G, Haynes PA (2012) Proteomics 12:906-21 CrossRef
  18. Leung PT, Wang Y, Maka SS, Ng WC, Leung KM (2011) Aquat Toxicol 105:49-1 CrossRef
  19. León IR, Neves-Ferreira AG, Valente RH, Mota EM, Lenzi HL, Perales J (2007) J Mass Spectrom 42:1363-374 CrossRef
  20. Regoli F, Cerrano C, Chierici E, Bompadre S, Bavestrello G (2000) Mar Biol 137:453-61 CrossRef
  21. Pribyl P, Cepák V, Zachleder V (2005) Protoplasma 226:231-40 CrossRef
  22. Lacoue-Labarthe T, Bihan L, Borg D, Koueta N, Bustamante P (2010) ICES J Mar Sci 67:1517-523
  23. Yan J, Wang J, Zhang H (2002) Plant J 29:193-02 CrossRef
  24. Hongsthong A, Sirijuntarut M, Yutthanasirikul R, Senachak J, Kurdrid P, Cheevadhanarak S, Tanticharoen M (2009) Prot Sci 7:33-2 CrossRef
  25. Morin S, Vivas-Nogues M, Duong TT, Boudou AM, Delmas F (2007) Fund Appl Limnol 168:179-87 CrossRef
  26. Rice-Evans CA, Miller NJ (1996) Biochem Soc Trans 24:790-95
  27. Jayaraman J, Namasivayam N (2011) Fund Clin Pharmacol 25:682-89 CrossRef
  28. Cao Z, Song P, Xu Q, Su R, Zhu G (2011) FEMS Microbiol Lett 320:9-4 CrossRef
  29. Decorosi F, Lori L, Santopolo L, Tatti E, Giovannetti L, Viti C (2011) Res Microbiol 162:747-55 CrossRef
  30. Gupta DK, Sandalio LM (2012) Metal toxicity in plants: perception, signaling and remediation. Springer, Germany CrossRef
  
• 作者单位：Rodrigo Cunha Wanick (1) (2) (5)
  Herbert de Sousa Barbosa (3) (5)
  Leonardo Revoredo Fraz?o (1)
  Ricardo Erthal Santelli (1) (2) (5)
  Marco Aurélio Zezzi Arruda (3) (5)
  Cristiano Carvalho Coutinho (4)
  
  1. Departamento de Geoquímica, Universidade Federal Fluminense, Outeiro S?o Jo?o Batista s/n, Centro, Niterói, RJ, 24020-150, Brazil
  2. Departamento de Química Analítica, Universidade Federal do Rio de Janeiro, Av. Athos da Silveira Ramos 149, Centro de Tecnologia, Bloco A, Cidade Universitária, Rio de Janeiro, RJ, 21941-909, Brazil
  5. INCT Bioanalítica, S?o Paulo, 13083-970, Brazil
  3. Grupo de Espectrometria, Preparo de Amostras e Mecaniza??o—GEPAM, Instituto de Química, Universidade Estadual de Campinas—UNICAMP, P.O. Box 6154, Campinas, SP, 13083-970, Brazil
  4. Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, 21941-902, Brazil
  
• ISSN：1618-2650

文摘

A comparative proteomic approach was used to assess differentially expressed proteins in marine sponges after 36?h of exposure to cadmium (Cd). After separation performed by 2-D polyacrylamide gel electrophoresis, 46 protein spots indicated differential expression, and 17 of these proteins were identified by electrospray ionization quadrupole time-of-flight mass spectrometry. From the proteins identified, 76?% were attributed to sponge-associated microorganisms (fungi and bacteria), and 24?% were attributed to Haliclona aquarius. Some of the proteins that were identified may be related to cell proliferation and differentiation or processes of oxidative stress repair and energy procurement. An integrated evaluation based on spot expression levels and the postulated functions of these proteins allowed a more accurate evaluation of the stress caused to the sponge holobiont system by cadmium exposure. This study could provide new insights into the use of a proteomic approach in the marine sponge to assess the effects of Cd pollution in a marine environment.