In Vivo Imaging of Lymph Node Migration of MNP- and 111In-Labeled Dendritic Cells in a Transgenic Mouse Model of Breast Cancer (MMTV-Ras)
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- 作者：Cristina Martelli (1) (2)
Manuela Borelli (3)
Luisa Ottobrini (1) (2)
Veronica Rainone (3)
Anna Degrassi (4)
Micaela Russo (4)
Umberto Gianelli (5)
Silvano Bosari (5)
Carlo Fiorini (6)
Daria Trabattoni (3)
Mario Clerici (2) (7) (8)
Giovanni Lucignani (1) (2) - 关键词：Dendritic cells ; MRI ; SPECT ; In vivo imaging ; Vaccine protocol ; Breast cancer
- 刊名：Molecular Imaging and Biology
- 出版年：2012
- 出版时间：April 2012
- 年：2012
- 卷：14
- 期：2
- 页码：183-196
- 全文大小：762KB
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Manuela Borelli (3)
Luisa Ottobrini (1) (2)
Veronica Rainone (3)
Anna Degrassi (4)
Micaela Russo (4)
Umberto Gianelli (5)
Silvano Bosari (5)
Carlo Fiorini (6)
Daria Trabattoni (3)
Mario Clerici (2) (7) (8)
Giovanni Lucignani (1) (2)
1. Department of Biomedical Sciences and Technologies, Section of Radiological Sciences, University of Milan, via di Rudinì 8, 20142, Milan, Italy
2. Centre of Molecular and Cellular Imaging -IMAGO, University of Milan, via Fratelli Cervi 93, 20090, Segrate, Milan, Italy
3. Department of Clinical Sciences, Chair of Immunology, University of Milan, via GB Grassi 74, Milan, Italy
4. Pharmacology Department, BU Oncology, Nerviano Medical Sciences, viale Pasteur 10, 20014, Nerviano, Milan, Italy
5. Pathology Unit, Department of Medicine, Surgery and Dentistry, University of Milan Medical School, IRCCS Ca-Granda -Ospedale Maggiore Policlinico Foundation, via Sforza, 28, 20122, Milan, Italy
6. Electronic and Information Technology Department, Politecnico di Milano, via Golgi 40, 20133, Milan and INFN, Milan Division, Milan, 20133, Italy
7. Department of Biomedical Sciences and Technologies, University of Milan, via Fratelli Cervi 93, 20090, Segrate, Milan, Italy
8. IRCCS Don C. Gnocchi Foundation, Via Capecelatro 66, 20148, Milan, Italy
文摘
Purpose The authors present a protocol for the in vivo evaluation, using different imaging techniques, of lymph node (LN) homing of tumor-specific dendritic cells (DCs) in a murine breast cancer model. Procedures Bone marrow DCs were labeled with paramagnetic nanoparticles (MNPs) or 111In-oxine. Antigen loading was performed using tumor lysate. Mature DCs were injected into the footpads of transgenic tumor-bearing mice (MMTV-Ras) and DC migration was tracked by magnetic resonance imaging (MRI) and single-photon emission computed tomography (SPECT). Ex vivo analyses were performed to validate the imaging data. Results DC labeling, both with MNPs and with 111In-oxine, did not affect DC phenotype or functionality. MRI and SPECT allowed the detection of iron and 111In in both axillary and popliteal LNs. Immunohistochemistry and γ-counting revealed the presence of DCs in LNs. Conclusions MRI and SPECT imaging, by allowing in vivo dynamic monitoring of DC migration, could further the development and optimization of efficient anti-cancer vaccines.