Psathyrostachys huashanica Keng (2n?=?2x?=?14, NsNs) from a cross between wheat cv. 7182 and P. huashanica via embryo culture, and we refer to this line as 3-8-10-2. We characterized this new line by cytology, genomic in situ hybridization (GISH), EST-SSR, EST-STS, and disease resistance screening. GISH using P. huashanica genomic DNA as the probe indicated that a pair of Ns chromosomes with strong hybridization signals was introduced into 3-8-10-2. We screened 255 EST-SSR and EST-STS multiple-loci markers from seven wheat homoeologous groups in the parent lines. Of these, 90 markers were polymorphic with a polymorphism frequency of 40?%, while two EST-SSR markers and six EST-STS markers located on wheat chromosome group 5 produced specific bands in P. huashanica and 3-8-10-2, respectively. This suggested that the introduced Ns chromosome pair belonged to homoeologous group 5, which was identified using new genome-specific markers. After inoculation with stripe rust isolates, 3-8-10-2 exhibited stripe rust resistance that probably originated from its P. huashanica parent. 3-8-10-2 can be used as a donor source for introducing novel disease resistance genes into wheat during breeding programs with the assistance of molecular and cytogenetic markers. Moreover, 3-8-10-2 had improved agronomic characteristics compared with its parents. Therefore, the addition line could be exploited as an important bridge for wheat breeding and chromosome engineering." />
Molecular cytogenetic identification of a wheat-em class="a-plus-plus">Psathyrostachys huashanica Keng 5Ns disomic addition line with stripe rust resistance
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  • 作者：Wanli Du (1)
    Jing Wang (1)
    Min Lu (1)
    Shugui Sun (1)
    Xinhong Chen (1)
    Jixin Zhao (1)
    Qunhui Yang (1)
    Jun Wu (1)
  • 关键词：Disomic addition line ; Homoeology ; Molecular cytogenetics ; Psathyrostachys huashanica ; Stripe rust ; Triticum aestivum
  • 刊名：Molecular Breeding
  • 出版年：2013
  • 出版时间：April 2013
  • 年：2013
  • 卷：31
  • 期：4
  • 页码：879-888
  • 全文大小：472KB
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  • 作者单位：Wanli Du (1)
    Jing Wang (1)
    Min Lu (1)
    Shugui Sun (1)
    Xinhong Chen (1)
    Jixin Zhao (1)
    Qunhui Yang (1)
    Jun Wu (1)

    1. Shaanxi Key Laboratory of Genetic Engineering for Plant Breeding, College of Agronomy, Northwest A&F University, Yangling, 712100, Shaanxi, China
  • ISSN：1572-9788
文摘
We developed a new stripe rust resistant line of common wheat-em class="a-plus-plus">Psathyrostachys huashanica Keng (2n?=?2x?=?14, NsNs) from a cross between wheat cv. 7182 and P. huashanica via embryo culture, and we refer to this line as 3-8-10-2. We characterized this new line by cytology, genomic in situ hybridization (GISH), EST-SSR, EST-STS, and disease resistance screening. GISH using P. huashanica genomic DNA as the probe indicated that a pair of Ns chromosomes with strong hybridization signals was introduced into 3-8-10-2. We screened 255 EST-SSR and EST-STS multiple-loci markers from seven wheat homoeologous groups in the parent lines. Of these, 90 markers were polymorphic with a polymorphism frequency of 40?%, while two EST-SSR markers and six EST-STS markers located on wheat chromosome group 5 produced specific bands in P. huashanica and 3-8-10-2, respectively. This suggested that the introduced Ns chromosome pair belonged to homoeologous group 5, which was identified using new genome-specific markers. After inoculation with stripe rust isolates, 3-8-10-2 exhibited stripe rust resistance that probably originated from its P. huashanica parent. 3-8-10-2 can be used as a donor source for introducing novel disease resistance genes into wheat during breeding programs with the assistance of molecular and cytogenetic markers. Moreover, 3-8-10-2 had improved agronomic characteristics compared with its parents. Therefore, the addition line could be exploited as an important bridge for wheat breeding and chromosome engineering.

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