Enhancement and modeling of microparticle-added Rhizopus oryzae lactic acid production

详细信息    查看全文

• 作者：Hasan Bugra Coban ; Ali Demirci
• 关键词：Lactic acid fermentation ; Rhizopus oryzae ; Microparticle ; Optimization ; Modeling
• 刊名：Bioprocess and Biosystems Engineering
• 出版年：2016
• 出版时间：February 2016
• 年：2016
• 卷：39
• 期：2
• 页码：323-330
• 全文大小：1,164 KB
• 参考文献：1.Branen AL, Davidson PM, Salminen S, Thorngate JH (2002) Food additives, 2nd edn. Marcel Dekker, NY
  2.Wee YJ, Kim JN, Ryu HW (2006) Biotechnological production of lactic acid and its recent applications. Food Technol Biotechnol 44:163–172
  3.Maas RHW, Eggink G, Bakker RR, Weusthuis RA (2006) Lactic acid production from xylose by the fungus Rhizopus oryzae. Appl Microbiol Biotechnol 72:861–868CrossRef
  4.Martinez FAC, Balciunas EM, Salgado JM, Gonzalez JMD, Converti A, Oliveira RPS (2013) Lactic acid properties, applications and production: a review. Trends Food Sci Tech 30:70–83CrossRef
  5.Rice AC, Pierson CS (1954) Factors influencing growth of Bacillus coagulans in canned tomato juice. II. Acidic constituents of tomato juice and specific organic acids. Food Res 19:124CrossRef
  6.Maneeboon T, Vanichsriratana W, Pomchaitaward C, Kitpreechavanich V (2010) Optimization of lactic acid production by pellet-form Rhizopus oryzae in 3-L airlift bioreactor using response surface methodology. Appl Biochem Biotechnol 161:137–146CrossRef
  7.Chotisubha-anandha N, Thitiprasert S, Thongchul N, Tolieng V (2011) Improved oxygen transfer and increased L-lactic acid production by morphology control of Rhizopus oryzae in a static bed bioreactor. Bioprocess Biosyst Eng 34:163–172CrossRef
  8.Kitpreechavanich V, Maneeboon T, Kayano Y, Sakai K (2008) Comparative characterization of l -lactic acid-producing thermotolerant Rhizopus fungi. J Biosci Bioeng 106:541–546CrossRef
  9.Vodnar DC, Dulf FV, Pop OL, Socaciu C (2013) L(+)-lactic acid production by pellet-form Rhizopus oryzae NRRL 395 on biodiesel crude glycerol. Microb Cell Fact 12:92CrossRef
  10.Wu X, Jiang S, Liu M, Pan L, Zheng Z, Luo S (2011) Production of L-lactic acid by Rhizopus oryzae using semicontinuous fermentation in bioreactor. J Ind Microbiol Biotechnol 38:565–571CrossRef
  11.Kaup BA, Ehrich K, Pescheck M, Schrader J (2008) Microparticle-enhanced cultivation of filamentous microorganisms: increased chloroperoxidase formation by Caldariomyces fumago as an example. Biotechnol Bioeng 99:491–498CrossRef
  12.Driouch H, Wittmann C, Hansch R, Wucherpfennig T, Krull R (2012) Improved enzyme production by bio-pellets Aspergillus niger: targeted morphology engineering using titanate microparticles. Biotechnol Bioeng 109:462–471CrossRef
  13.Driouch H, Roth A, Dersch P, Wittmann C (2011) Filamentous fungi in good shape microparticles for tailor-made fungal morphology and enhanced enzyme production. Bioeng Bugs 2:100–104CrossRef
  14.Coban HB, Demirci A, Turhan I (2015) Microparticle-enhanced Aspergillus ficuum phytase production and evaluation of fungal morphology in submerged fermentation. Bioprocess Biosyst Eng 38:1075–1080CrossRef
  15.Zwietering MH, Jongenburger I, Rombouts FM, Rient KV (1990) Modeling of the bacterial growth curve. Appl Environ Microbiol 56:1875–1881
  16.Park EY, Kosakai Y, Okabe M (1998) Efficient production of L-(+)-lactic acid using mycelial cotton-like flocs of Rhizopus oryzae in an air-lift bioreactor. Biotechnol Prog 14:699–704CrossRef
  17.Zhang ZY, Jin B, Kelly JM (2007) Production of lactic acid from renewable materials by Rhizopus fungi. Biochem Eng J 35:251–263CrossRef
  
• 作者单位：Hasan Bugra Coban (1)
  Ali Demirci (1) (2)
  
  1. Department of Agricultural and Biological Engineering, The Pennsylvania State University, University Park, PA, 16802, USA
  2. The Huck Institutes of Life Sciences, The Pennsylvania State University, University Park, PA, 16802, USA
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Biotechnology
  Industrial Chemistry and Chemical Engineering
  Industrial and Production Engineering
  Waste Management and Waste Technology
  Waste Water Technology, Water Pollution Control, Water Management and Aquatic Pollution
  Food Science
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1615-7605

文摘

Lactic acid has a wide industrial application area and can be produced by fungal strains. However, excessive bulk growth form of fungi during the fermentations is a major problem, which limits the fermentation performance. Microparticles are excellent tools to prevent bulk fungal growth and provide homogenized fermentation broth to increase uniformity and the prediction performance of the models. Therefore, in this study, addition of aluminum oxide and talcum microparticles into fermentations was evaluated to enhance the production of lactic acid by Rhizopus oryzae. The results showed that the bulk fungal growth was prevented and the lactic acid concentration increased from 6.02 to 13.88 and 24.01 g/L, when 15 g/L of aluminum oxide or 10 g/L of talcum was used, respectively, in the shake-flask fermentations. Additionally, substrate concentration, pH, and agitation were optimized in the bioreactors using response surface methodology, and optimum values were determined as 126 g/L of glucose, 6.22 pH, and 387 rpm, respectively. Under these conditions, lactic acid production further increased to 75.1 ± 1.5 g/L with 10 g/L of talcum addition. Also, lactic acid production and glucose consumption in the batch fermentation were successfully modeled with modified Gompertz model and modified logistic model. RMSE and MAE values for lactic acid production were calculated as 2.279 and 1.498 for the modified Gompertz model; 3.6 and 4.056 for the modified logistic model. Additionally, modified logistic model predicted glucose consumption with −2.088 MAE and 2.868 RMSE, whereas these values were calculated as 2.035 and 3.946 for the modified Gompertz model. Keywords Lactic acid fermentation Rhizopus oryzae Microparticle Optimization Modeling