Identification of potential biomarkers for xylene exposure by microarray analyses of gene expression and methylation

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• 作者：Seol Young Kim ; Ji Young Hong ; So-Yeon Yu ; Gi Won Kim…
• 关键词：Xylene ; Microarray ; Gene expression ; Methylation ; Biomarker
• 刊名：Molecular & Cellular Toxicology
• 出版年：2016
• 出版时间：March 2016
• 年：2016
• 卷：12
• 期：1
• 页码：15-20
• 全文大小：787 KB
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• 作者单位：Seol Young Kim (1)
  Ji Young Hong (1)
  So-Yeon Yu (2)
  Gi Won Kim (2)
  Jeong Jin Ahn (1)
  Youngjoo Kim (1)
  Sang Wook Son (3)
  Jong-Tae Park (4)
  Seung Yong Hwang (1) (2)
  
  1. Department of Bio-Nanotechnology, Hanyang University, Sangnok-gu, Ansan, Gyeonggi-do, Korea
  2. Department of Molecular & Life Science, Hanyang University, Sangnok-gu, Ansan, Gyeonggi-do, Korea
  3. Department of Dermatology, Korea University Medical Center, Seoul, Korea
  4. Department of Occupational and Environmental Medicine, Korea University Ansan Hospital, Korea University College of Medicine, Danwon-gu, Ansan, Gyeonggi-do, Korea
  
• 刊物主题：Cell Biology; Pharmacology/Toxicology;
• 出版者：Springer Netherlands
• ISSN：2092-8467

文摘

Xylene is volatile organic compound that has been reported to increase the incidence of cancer and various diseases related to the immune system, cardiovascular systems, respiratory and reproductive organs. However, there are currently few biomarkers in human cases. Using microarray, we analysed 10 participants for the xylene-exposure group and 10 controls that were not exposed to xylene. The two groups were compared in terms of expression levels and methylation patterns. We identified 6 genes that were down-regulated and hyper-methylated, and 132 that were up-regulated and hypo-methylated in the xylene- exposure group compared to control. We sorted out and 28 biomarker candidates were chosen using DAVID. And then, we used IPA to select the significant potential biomarkers in them. We used network analysis and selected 6 significant genes, and these 6 genes showed altered expression and methylation in xylene-exposure group, suggesting that they are suitable potential biomarkers for xylene exposure.