Development and characterization of 13 polymorphic microsatellite markers for the Chinese surf clam (Mactra chinensis) through Illumina paired-end sequencing

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• 作者：Hongjun Li (1)
  Jingjing Zhang (1)
  Hongbo Li (1)
  Xianggang Gao (2)
  Chongbo He (2)
  
• 关键词：Mactra chinensis ; Microsatellite ; SSR ; Transcriptome sequencing
• 刊名：Conservation Genetics Resources
• 出版年：2014
• 出版时间：December 2014
• 年：2014
• 卷：6
• 期：4
• 页码：877-879
• 全文大小：153 KB
• 参考文献：1. Grabherr MG, Haas BJ, Yassour M, Levin JZ, Thompson DA, Amit I et al (2011) Full-length transcriptome assembly from RNA-Seq data without a reference genome. Nat Biotechnol 29:644-52 CrossRef
  2. Li Q, Wan JM (2005) SSRHUNTER: development of a local searching software for SSR sites. Yichuan 27:808-10
  3. Ni LH, Li Q, Kong LF (2010) Isolation and characterization of 19 microsatellite markers from the Chinese surf clam ( / Mactra chinensis). Conserv Genet Resour 2:27-0 CrossRef
  4. Xing K, Gao ML, Li HJ (2014) Genetic differentiation between natural and hatchery populations of Manila clam ( / Ruditapes philippinarum) based on microsatellite markers. Genet Mol Res 13:237-45 CrossRef
  5. Yeh FC, Yang R, Boyle TJ, Ye Z, Xiyan JM (2000) POPGENE 32. Molecular Biology and Biotechnology Centre, University of Alberta, Edmonton, Microsoft Windows-based freeware for population genetic analysis
  
• 作者单位：Hongjun Li (1)
  Jingjing Zhang (1)
  Hongbo Li (1)
  Xianggang Gao (2)
  Chongbo He (2)
  
  1. Key Laboratory for Ecological Environment in Coastal Areas (SOA), National Marine Environmental Monitoring Center, Dalian, 116023, China
  2. Liaoning Ocean and Fisheries Science Research Institute, Dalian, 116023, China
  
• ISSN：1877-7260

文摘

Chinese surf clam (Mactra chinensis) is a commercially important bivalve in China. The high-throughout transcriptome sequencing of M. chinensis provided a valuable resource for mining large number of microsatellite markers. In this study, we developed and characterized 13 novel polymorphic microsatellite markers for M. chinensis. The number of alleles varied from 4 to 7, with an average of 5.2 per locus. The observed and expected heterozygosity ranged from 0.1667 to 0.7368 and 0.6434 to 0.8056, respectively. Two loci significantly deviated from Hardy–Weinberg equilibrium after Bonferroni correction. These novel informative microsatellite markers will be useful for further genetic diversity analysis, linkage mapping, evolutionary studies and marker-assisted breeding.