A novel hybrid baculovirus-adeno-associated viral vector-mediated radionuclide reporter gene imaging system for stem cells transplantation monitoring

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• 作者：Yu Pan ; Hongyan Yin ; Jing Lv ; Huijun Ju…
• 关键词：Baculovirus ; Hybrid virus ; Inverted terminal repeat ; Mesenchymal stem cells ; Radionuclide reporter gene ; Sodium ; iodine symporter
• 刊名：Applied Microbiology and Biotechnology
• 出版年：2015
• 出版时间：February 2015
• 年：2015
• 卷：99
• 期：3
• 页码：1415-1426
• 全文大小：2,162 KB
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• 作者单位：Yu Pan (1)
  Hongyan Yin (2)
  Jing Lv (1)
  Huijun Ju (1)
  Xiang Zhou (3)
  Yifan Zhang (1)
  
  1. Department of Nuclear Medicine, Rui Jin Hospital, Shanghai Jiao Tong University School of Medicine, No. 197, Ruijin 2nd Road, Shanghai, 200025, China
  2. Department of Nuclear Medicine, Zhong Shan Hospital, Fudan University, No. 180, Fenglin Road, Shanghai, 200032, China
  3. Department of Nuclear Medicine, Renji Hospital, Shanghai Jiao Tong University School of Medicine, No. 1630, Dongfang Road, Shanghai, 200127, China
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Biotechnology
  Microbiology
  Microbial Genetics and Genomics
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1432-0614

文摘

Hybrid baculovirus-adeno-associated virus (BV-AAV) containing enhanced green fluorescent protein (eGFP) reporter gene or human sodium-iodide symporter (hNIS) reporter gene flanked by inverted terminal repeats (ITRs) derived from AAV (BV-CMV-eGFP-ITR and BV-CMV-hNIS-ITR) were constructed and used to investigate the feasibility of using hybrid BV-AAV transgenic vector to mediate hNIS reporter gene imaging for monitoring bone marrow-derived mesenchymal stem cells (BM-MSCs) transplantation therapy as a novel biotechnological platform in radionuclide reporter gene?imaging. The results showed that the infection efficiency of BV-CMV-eGFP-ITR in BM-MSCs reached 84.25?±-.38?%, and there were no obvious adverse effects on BM-MSCs. The 125I?/sup> and 99mTcO4 ?/sup> uptake assays showed that the radionuclide accumulation induced by BA-AAV-mediated hNIS was highly efficient in infected BM-MSCs. Furthermore, there was a robust correlation between the infected BM-MSCs cell number and the 125I?/sup> accumulation amount (R 2--.9026). The micro-SPECT/CT imaging showed that BV-CMV-hNIS-ITR-infected BM-MSCs accumulated radioiodine efficiently in vivo, exhibiting obvious radiotracer accumulation in transplantation sites. Further quantitative analysis revealed that 30?min might be the optimal imaging time point. Moreover, the revealed high target/individual organ background ratios also supported the feasibility of BV-AAV-mediated hNIS reporter gene imaging for monitoring BM-MSCs transplantation in most of commonly used transplantation sites, thus highlighting this promise biotechnological platform in radionuclide reporter gene imaging for stem cell transplantation therapy.