Complete genomic sequence of a Pineapple mealybug wilt-associated virus-1 from Hainan Island, China

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• 作者：Naitong Yu ; Zhiwen Luo ; Hongyan Fan ; Zhili Zhang…
• 关键词：Genome ; Pineapple mealybug wilt ; associated virus ; 1 ; Sequencing
• 刊名：European Journal of Plant Pathology
• 出版年：2015
• 出版时间：March 2015
• 年：2015
• 卷：141
• 期：3
• 页码：611-615
• 全文大小：531 KB
• 参考文献：1. Gambley, C. F., Steele, V., Geering, A. D. W., & Thomas, J. E. (2008). The genetic diversity of ampeloviruses in Australian pineapples and their association with mealybug wilt disease. / Australasian Plant Pathology, 37, 95-05. CrossRef
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• 作者单位：Naitong Yu (1) (2)
  Zhiwen Luo (1)
  Hongyan Fan (1)
  Zhili Zhang (1)
  Xianghong Li (1)
  Jianhua Wang (2)
  Zhixin Liu (2)
  Fan He (1)
  
  1. Institute of Tropical Fruit Tree, Hainan Academy of Agricultural Science/Haikou Investigation Station of Tropical Fruit Trees, Ministry of Agriculture, Hainan, Haikou, 571100, China
  2. Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan, 571101, China
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Plant Pathology
  Plant Sciences
  Ecology
  
• 出版者：Springer Netherlands
• ISSN：1573-8469

文摘

This is the first report of the complete genome sequence of Pineapple mealybug wilt-associated virus-1 (PMWaV-1 HN, KJ872494) identified on pineapple [Ananas comosus (L.) Merr.] in Hainan island, China. The genome is 13,069 nucleotides long, possessing seven open reading frames (ORFs). The ORF1 encodes a 233?kDa polyprotein which has PRO protease domain, MTR methyltransferase domain and HEL helicase domain; The ORF2?~?ORF7 encode RNA-dependent RNA polymerase protein (RdRp), a 6?kDa protein (p6), heat shock 70 homolog protein (HSP70h), a 46?kDa protein (p46), coat protein (CP) and a 24?kDa protein (p24), respectively. Full-length nucleotide sequence analysis of PMWaV-1 HN genome confirmed it 97.99?% homology with PMWaV-1 (AF414119) which was isolated from Hawaii, USA. Furthermore, based on Maximum Likelihood method, all conserved trees of RdRp, HSP70h and CP proteins sequences?revealed that PMWaV-1 HN together with other PMWaV-1 isolates clearly separated into PMWaV-1 group.