Enhancement of D-lactic acid production from a mixed glucose and xylose substrate by the Escherichia coli strain JH15 devoid of the glucose effect
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- 作者：Hongying Lu ; Xiao Zhao ; Yongze Wang ; Xiaoren Ding ; Jinhua Wang…
- 关键词：Catabolite repression ; D ; lactic acid ; E. coli ; Glucose effect ; ptsG ; PLA ; Xylose fermentation
- 刊名：BMC Biotechnology
- 出版年：2016
- 出版时间：December 2016
- 年：2016
- 卷：16
- 期：1
- 全文大小：889 KB
- 刊物类别：Chemistry and Materials Science
- 刊物主题：Biotechnology
Life Sciences
Plant Breeding/Biotechnology
Stem Cells
Transgenics - 出版者：BioMed Central
- ISSN：1472-6750
文摘
Background A thermal tolerant stereo-complex poly-lactic acid (SC-PLA) can be made by mixing Poly-D-lactic acid (PDLA) and poly-L-lactic acid (PLLA) at a defined ratio. This environmentally friendly biodegradable polymer could replace traditional recalcitrant petroleum-based plastics. To achieve this goal, however, it is imperative to produce optically pure lactic acid isomers using a cost-effective substrate such as cellulosic biomass. The roadblock of this process is that: 1) xylose derived from cellulosic biomass is un-fermentable by most lactic acid bacteria; 2) the glucose effect results in delayed and incomplete xylose fermentation. An alternative strain devoid of the glucose effect is needed to co-utilize both glucose and xylose for improved D-lactic acid production using a cellulosic biomass substrate.