Ectopic expression of RBP4 impairs the insulin pathway and inguinal fat deposition in mice

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• 作者：Jia Cheng (1) (2)
  Yuefeng Li (1)
  Guofang Wu (1)
  Jiameng Zheng (1)
  Hongzhao Lu (1) (2)
  Xin’e Shi (1)
  Gongshe Yang (1)
  
• 关键词：RBP4 ; Adipokine ; Inguinal fat deposition ; Insulin sensitivity
• 刊名：Journal of Physiology and Biochemistry
• 出版年：2014
• 出版时间：June 2014
• 年：2014
• 卷：70
• 期：2
• 页码：479-486
• 全文大小：
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• 作者单位：Jia Cheng (1) (2)
  Yuefeng Li (1)
  Guofang Wu (1)
  Jiameng Zheng (1)
  Hongzhao Lu (1) (2)
  Xin’e Shi (1)
  Gongshe Yang (1)
  
  1. College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, 712100, China
  2. Vitamin D Research Institute, Shaanxi University of Technology, Hanzhong, Shaanxi, 723000, China
  
• ISSN：1877-8755

文摘

A large body of evidence has linked retinol-binding protein 4 (RBP4) to systemic insulin resistance, but little is known about its function in fat deposition. This study aimed to confirm the involvement of RBP4 in inguinal fat deposition and insulin by intraperitoneal injection of adenovirus-mediated RBP4 to mice. Intraperitoneal injection of adenoviral vectors was validated as an efficient gene manipulation tool for over-expressing recombinant proteins in vivo. Ectopic expression of RBP4 decelerated inguinal fat deposition by decreasing the size of adipocytes. Moreover, the introduction of exogenous RBP4 blunted the response of inguinal adipocytes to insulin signals. These findings suggest that RBP4 impaired in vivo adipogenesis, partly through the repression of the insulin pathway.