Genetical identification of coccidia in red-crowned crane, Grus japonensis

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• 作者：Hajime Honma ; Tetsushi Yokoyama ; Masako Inoue ; Akiko Uebayashi ; Fumio Matsumoto ; Yuki Watanabe and Yutaka Nakai
• 刊名：Parasitology Research
• 出版年：2007
• 出版时间：February, 2007
• 年：2007
• 卷：100
• 期：3
• 页码：637-640
• 全文大小：212 KB
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Biomedicine
  Medical Microbiology
  Microbiology
  Immunology
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1432-1955

文摘

We genetically analyzed eimerian oocysts isolated from the red-crowned crane (Grus japonensis) in Hokkaido, a northern island of Japan. Two types of oocysts of which shapes were similar to Eimeria gruis and E. reichenowi were found. Nearly the total length of the 18S ribosomal RNA gene (about 1.7 kbp in length) was amplified from single oocyst of each type and was sequenced. The respective sequences showed high similarity to those of published partial sequences (349 bp) of E. gruis type oocyst and E. reichenowi type oocyst isolated from the hooded crane (G. monacha) and the white-naped crane (G. vipio) in Izumi, in the southern island of Japan. Phylogenetic analysis indicated that E. gruis type and E. reichenowi type are different species, and suggested that these crane coccidia have evolved independently from the intestinal parasitizing Eimeria species.