Gene expression of transporters and phase I/II metabolic enzymes in murine small intestine during fasting
详细信息 查看全文
- 作者：Heleen M van den Bosch (1) (2)
Meike Bünger (1) (2)
Philip J de Groot (1) (2)
Jolanda van der Meijde (1)
Guido JEJ Hooiveld (1) (2)
Michael Müller (1) (2) - 刊名：BMC Genomics
- 出版年：2007
- 出版时间：December 2007
- 年：2007
- 卷：8
- 期：1
- 全文大小：350KB
- 参考文献：1. van den Berghe G: The role of the liver in metabolic homeostasis: implications for inborn errors of metabolism. / J Inherit Metab Dis 1991,14(4):407-20. CrossRef
2. Kersten S, Seydoux J, Peters JM, Gonzalez FJ, Desvergne B, Wahli W: Peroxisome proliferator-activated receptor alpha mediates the adaptive response to fasting. / J Clin Invest 1999,103(11):1489-498. CrossRef
3. Stenberg P, Luthman K, Artursson P: Virtual screening of intestinal drug permeability. / J Control Release 2000,65(1-):231-43. CrossRef
4. Lee VH: Membrane transporters. / Eur J Pharm Sci 2000, 11 Suppl 2:S41-0. CrossRef
5. Steffansen B, Nielsen CU, Brodin B, Eriksson AH, Andersen R, Frokjaer S: Intestinal solute carriers: an overview of trends and strategies for improving oral drug absorption. / Eur J Pharm Sci 2004,21(1):3-6. j.ejps.2003.10.010">CrossRef
6. Tsuji A, Tamai I: Carrier-mediated intestinal transport of drugs. / Pharm Res 1996,13(7):963-77. CrossRef
7. Anderle P, Huang Y, Sadee W: Intestinal membrane transport of drugs and nutrients: genomics of membrane transporters using expression microarrays. / Eur J Pharm Sci 2004,21(1):17-4. CrossRef
8. Hediger MA, Romero MF, Peng JB, Rolfs A, Takanaga H, Bruford EA: The ABCs of solute carriers: physiological, pathological and therapeutic implications of human membrane transport proteins. / Pflugers Arch 2004,447(5):465-68. CrossRef
9. Wacher VJ, Salphati L, Benet LZ: Active secretion and enterocytic drug metabolism barriers to drug absorption. / Adv Drug Deliv Rev 2001,46(1-):89-02. CrossRef
10. Kaminsky LS, Zhang QY: The small intestine as a xenobiotic-metabolizing organ. / Drug Metab Dispos 2003,31(12):1520-525. CrossRef
11. Danielson PB: The cytochrome P450 superfamily: biochemistry, evolution and drug metabolism in humans. / Curr Drug Metab 2002,3(6):561-97. CrossRef
12. Banoglu E: Current status of the cytosolic sulfotransferases in the metabolic activation of promutagens and procarcinogens. / Curr Drug Metab 2000,1(1):1-0. CrossRef
13. Weinshilboum RM, Otterness DM, Aksoy IA, Wood TC, Her C, Raftogianis RB: Sulfation and sulfotransferases 1: Sulfotransferase molecular biology: cDNAs and genes. / FASEB J 1997,11(1):3-4.
14. Tukey RH, Strassburg CP: Human UDP-Glucuronosyltransferases: Metabolism, Expression, and Disease. / Annual Review of Pharmacology and Toxicology 2000,40(1):581-16. CrossRef
15. Tew KD, Ronai Z: GST function in drug and stress response. / Drug Resistance Updates 1999,2(3):143. CrossRef
16. Moscow JA, Dixon KH: Glutathione-related enzymes, glutathione and multidrug resistance. / Cytotechnology 1993,12(1-):155-70. CrossRef
17. Vatsis KP, Weber WW, Bell DA, Dupret JM, Evans DA, Grant DM, Hein DW, Lin HJ, Meyer UA, Relling MV, / et al.: Nomenclature for N-acetyltransferases. / Pharmacogenetics 1995,5(1):1-7. CrossRef
18. Arand M, Cronin A, Adamska M, Oesch F: Epoxide hydrolases: structure, function, mechanism, and assay. / Methods Enzymol 2005, 400:569-88. CrossRef
19. Borst P, Elferink RO: Mammalian ABC transporters in health and disease. / Annu Rev Biochem 2002, 71:537-92. CrossRef
20. Ferraris RP, Carey HV: Intestinal transport during fasting and malnutrition. / AnnuRevNutr 2000, 20:195-19.
21. Miyauchi S, Gopal E, Fei YJ, Ganapathy V: Functional identification of SLC5A8, a tumor suppressor down-regulated in colon cancer, as a Na(+)-coupled transporter for short-chain fatty acids. / J Biol Chem 2004,279(14):13293-3296. jbc.C400059200">CrossRef
22. Markovich D, Murer H: The SLC13 gene family of sodium sulphate/carboxylate cotransporters. / Pflugers Arch 2004,447(5):594-02. CrossRef
23. Endo S, Nomura T, Chan BS, Lu R, Pucci ML, Bao Y, Schuster VL: Expression of PGT in MDCK cell monolayers: polarized apical localization and induction of active PG transport. / Am J Physiol Renal Physiol 2002,282(4):F618-22.
24. Mount DB, Romero MF: The SLC26 gene family of multifunctional anion exchangers. / Pflugers Arch 2004,447(5):710-21. CrossRef
25. Eide DJ: The SLC39 family of metal ion transporters. / Pflugers Arch 2004,447(5):796-00. CrossRef
26. Gill RK, Saksena S, Alrefai WA, Sarwar Z, Goldstein JL, Carroll RE, Ramaswamy K, Dudeja PK: Expression and membrane localization of MCT isoforms along the length of the human intestine. / Am J Physiol Cell Physiol 2005,289(4):C846-52. jpcell.00112.2005">CrossRef
27. McKie AT, Barlow DJ: The SLC40 basolateral iron transporter family (IREG1/ferroportin/MTP1). / Pflugers Arch 2004,447(5):801-06. CrossRef
28. Martel F: Recent advances on the importance of the serotonin transporter SERT in the rat intestine. / Pharmacol Res 2006,54(2):73-6. j.phrs.2006.04.005">CrossRef
29. Liuzzi JP, Cousins RJ: Mammalian zinc transporters. / Annu Rev Nutr 2004, 24:151-72. CrossRef
30. Bartoloni L, Antonarakis SE: The human sugar-phosphate/phosphate exchanger family SLC37. / Pflugers Arch 2004,447(5):780-83. CrossRef
31. Steinberg SJ, Wang SJ, Kim DG, Mihalik SJ, Watkins PA: Human very-long-chain acyl-CoA synthetase: cloning, topography, and relevance to branched-chain fatty acid metabolism. / Biochem Biophys Res Commun 1999,257(2):615-21. CrossRef
32. Kamiyama S, Sasaki N, Goda E, Ui-Tei K, Saigo K, Narimatsu H, Jigami Y, Kannagi R, Irimura T, Nishihara S: Molecular Cloning and Characterization of a Novel 3'-Phosphoadenosine 5'-Phosphosulfate Transporter, PAPST2. / J Biol Chem 2006,281(16):10945-0953. jbc.M508991200">CrossRef
33. Palmieri F: The mitochondrial transporter family (SLC25): physiological and pathological implications. / Pflugers Arch 2004,447(5):689-09. CrossRef
34. Ishida N, Kawakita M: Molecular physiology and pathology of the nucleotide sugar transporter family (SLC35). / Pflugers Arch 2004,447(5):768-75. CrossRef
35. Alberti S, Schuster G, Parini P, Feltkamp D, Diczfalusy U, Rudling M, Angelin B, Bjorkhem I, Pettersson S, Gustafsson JA: Hepatic cholesterol metabolism and resistance to dietary cholesterol in LXR{beta}-deficient mice. / J Clin Invest 2001,107(5):565-73. CrossRef
36. Lund E, Andersson O, Zhang J, Babiker A, Ahlborg G, Diczfalusy U, Einarsson K, Sjovall J, Bjorkhem I: Importance of a Novel Oxidative Mechanism for Elimination of Intracellular Cholesterol in Humans. / Arterioscler Thromb Vasc Biol 1996,16(2):208-12.
37. Jia G, Takahashi R, Zhang Z, Tsuji Y, Sone H: Aldo-keto reductase 1 family B7 is the gene induced in response to oxidative stress in the livers of Long-Evans Cinnamon rats. / Int J Oncol 2006,29(4):829-38.
38. Wang N, Silver DL, Thiele C, Tall AR: ATP-binding Cassette Transporter A1 (ABCA1) Functions as a Cholesterol Efflux Regulatory Protein. / J Biol Chem 2001,276(26):23742-3747. jbc.M102348200">CrossRef
39. Yu L, Li-Hawkins J, Hammer RE, Berge KE, Horton JD, Cohen JC, Hobbs HH: Overexpression of ABCG5 and ABCG8 promotes biliary cholesterol secretion and reduces fractional absorption of dietary cholesterol. / J Clin Invest 2002,110(5):671-80.
40. Kislinger T, Cox B, Kannan A, Chung C, Hu P, Ignatchenko A, Scott MS, Gramolini AO, Morris Q, Hallett MT, Rossant J, Hughes TR, Frey B, Emili A: Global survey of organ and organelle protein expression in mouse: combined proteomic and transcriptomic profiling. / Cell 2006,125(1):173-86. j.cell.2006.01.044">CrossRef
41. Cox B, Kislinger T, Wigle DA, Kannan A, Brown K, Okubo T, Hogan B, Jurisica I, Frey B, Rossant J, Emili A: Integrated proteomic and transcriptomic profiling of mouse lung development and Nmyc target genes. / Mol Syst Biol 2007., 3:
42. Lenaerts K, Sokolovic M, Bouwman FG, Lamers WH, Mariman EC, Renes J: Starvation induces phase-specific changes in the proteome of mouse small intestine. / J Proteome Res 2006,5(9):2113-122. CrossRef
43. Chappell VL, Thompson MD, Jeschke MG, Chung DH, Thompson JC, Wolf SE: Effects of incremental starvation on gut mucosa. / Dig Dis Sci 2003,48(4):765-69. CrossRef
44. Samsom M, Fraser R, Smout AJ, Verhagen MA, Adachi K, Horowitz M, Dent J: Characterization of small intestinal pressure waves in ambulant subjects recorded with a novel portable manometric system. / Dig Dis Sci 1999,44(11):2157-164. CrossRef
45. Lordal M, Wallen H, Hjemdahl P, Beck O, Hellstrom PM: Concentration-dependent stimulation of intestinal phase III of migrating motor complex by circulating serotonin in humans. / Clin Sci (Lond) 1998,94(6):663-70.
46. Liu MC, Sakakibara Y, Liu CC: Bacterial expression, purification, and characterization of a novel mouse sulfotransferase that catalyzes the sulfation of eicosanoids. / Biochem Biophys Res Commun 1999,254(1):65-9. CrossRef
47. Makkar RS, Contreras MA, Paintlia AS, Smith BT, Haq E, Singh I: Molecular organization of peroxisomal enzymes: protein-protein interactions in the membrane and in the matrix. / Arch Biochem Biophys 2006,451(2):128-40. j.abb.2006.05.003">CrossRef
48. Pajor AM: Sodium-coupled transporters for Krebs cycle intermediates. / Annu Rev Physiol 1999, 61:663-82. CrossRef
49. Venkateswaran A, Laffitte BA, Joseph SB, Mak PA, Wilpitz DC, Edwards PA, Tontonoz P: Control of cellular cholesterol efflux by the nuclear oxysterol receptor LXRalpha. / PNAS 2000,97(22):12097-2102. CrossRef
50. Repa JJ, Berge KE, Pomajzl C, Richardson JA, Hobbs H, Mangelsdorf DJ: Regulation of ATP-binding Cassette Sterol Transporters ABCG5 and ABCG8 by the Liver X Receptors alpha and beta. / J Biol Chem 2002,277(21):18793-8800. jbc.M109927200">CrossRef
51. Fu X, Menke JG, Chen Y, Zhou G, MacNaul KL, Wright SD, Sparrow CP, Lund EG: 27-Hydroxycholesterol Is an Endogenous Ligand for Liver X Receptor in Cholesterol-loaded Cells. / J Biol Chem 2001,276(42):38378-8387. jbc.M105805200">CrossRef
52. Bauer M, Hamm AC, Bonaus M, Jacob A, Jaekel J, Schorle H, Pankratz MJ, Katzenberger JD: Starvation response in mouse liver shows strong correlation with life-span-prolonging processes. / Physiol Genomics 2004,17(2):230-44. CrossRef
53. Guengerich FP: Cytochrome P-450 3A4: regulation and role in drug metabolism. / Annu Rev Pharmacol Toxicol 1999, 39:1-7. CrossRef
54. Townsend DM, Tew KD, Tapiero H: The importance of glutathione in human disease. / Biomed Pharmacother 2003,57(3-):145-55. CrossRef
55. Battiston L, Moretti M, Tulissi P, Micheli L, Marchi P, Mazzoran L, Lunazzi G, Pozzato G: Hepatic glutathione determination after ethanol administration in rat: evidence of the first-pass metabolism of ethanol. / Life Sci 1995,56(4):241-48. CrossRef
56. Szkudelski T, Okulicz M, Bialik I, Szkudelska K: The influence of fasting on liver sulfhydryl groups, glutathione peroxidase and glutathione-S-transferase activities in the rat. / J Physiol Biochem 2004,60(1):1-. CrossRef
57. Di Simplicio P, Rossi R, Falcinelli S, Ceserani R, Formento ML: Antioxidant status in various tissues of the mouse after fasting and swimming stress. / Eur J Appl Physiol Occup Physiol 1997,76(4):302-07. CrossRef
58. Bartlett K, Eaton S: Mitochondrial {beta}-oxidation. / Eur J Biochem 2004,271(3):462-69. j.1432-1033.2003.03947.x">CrossRef
59. Beutler E, Gelbart T, Pegelow C: Erythrocyte glutathione synthetase deficiency leads not only to glutathione but also to glutathione-S-transferase deficiency. / J Clin Invest 1986,77(1):38-1. CrossRef
60. Kensler TW, Wakabayashi N, Biswal S: Cell Survival Responses to Environmental Stresses Via the Keap1-Nrf2-ARE Pathway. / Annual Review of Pharmacology and Toxicology 2007,47(1):89-16. CrossRef
61. Patsouris D, Reddy JK, Muller M, Kersten S: Peroxisome proliferator-activated receptor alpha mediates the effects of high-fat diet on hepatic gene expression. / Endocrinology 2006,147(3):1508-516. CrossRef
62. Kok T, Wolters H, Bloks VW, Havinga R, Jansen PL, Staels B, Kuipers F: Induction of hepatic ABC transporter expression is part of the PPARalpha-mediated fasting response in the mouse. / Gastroenterology 2003,124(1):160-71. CrossRef
63. Vamecq J, Latruffe N: Medical significance of peroxisome proliferator-activated receptors. / Lancet 1999,354(9173):141-48. CrossRef
64. Knight BL, Patel DD, Humphreys SM, Wiggins D, Gibbons GF: Inhibition of cholesterol absorption associated with a PPAR alpha-dependent increase in ABC binding cassette transporter A1 in mice. / J Lipid Res 2003,44(11):2049-058. jlr.M300042-JLR200">CrossRef
65. Santamarina-Fojo S, Peterson K, Knapper C, Qiu Y, Freeman L, Cheng JF, Osorio J, Remaley A, Yang XP, Haudenschild C, Prades C, Chimini G, Blackmon E, Francois T, Duverger N, Rubin EM, Rosier M, Denefle P, Fredrickson DS, Brewer HB Jr.: Complete genomic sequence of the human ABCA1 gene: analysis of the human and mouse ATP-binding cassette A promoter. / Proc Natl Acad Sci U S A 2000,97(14):7987-992. CrossRef
66. Lee SS, Pineau T, Drago J, Lee EJ, Owens JW, Kroetz DL, Fernandez-Salguero PM, Westphal H, Gonzalez FJ: Targeted disruption of the alpha isoform of the peroxisome proliferator-activated receptor gene in mice results in abolishment of the pleiotropic effects of peroxisome proliferators. / Mol Cell Biol 1995,15(6):3012-022.
67. Gentleman RC, Carey VJ, Bates DM, Bolstad B, Dettling M, Dudoit S, Ellis B, Gautier L, Ge Y, Gentry J, Hornik K, Hothorn T, Huber W, Iacus S, Irizarry R, Leisch F, Li C, Maechler M, Rossini AJ, Sawitzki G, Smith C, Smyth G, Tierney L, Yang JY, Zhang J: Bioconductor: open software development for computational biology and bioinformatics. / Genome Biol 2004,5(10):R80. CrossRef
68. Wu Z, Irizarry RA, Gentleman R, Martinez-Murillo F, Spencer F: A Model-Based Background Adjustment for Oligonucleotide Expression Arrays. / Journal of the American Statistical Association 2004,99(468):909-17. CrossRef
69. Smyth GK: Limma: linear models for microarray data. / Bioinformatics and Computational Biology Solutions using R and Bioconductor / (Edited by: R. Gentleman VCSDRIWH). New York, Springer 2005, 1:397-20. CrossRef
70. Kendziorski CM, Newton MA, Lan H, Gould MN: On parametric empirical Bayes methods for comparing multiple groups using replicated gene expression profiles. / Stat Med 2003,22(24):3899-914. CrossRef
71. Kooperberg C, Aragaki A, Strand AD, Olson JM: Significance testing for small microarray experiments. / Stat Med 2005,24(15):2281-298. CrossRef
72. Gottardo R, Pannucci JA, Kuske CR, Brettin T: Statistical analysis of microarray data: a Bayesian approach. / Biostatistics 2003,4(4):597-20. CrossRef
73. Smyth GK: Linear models and empirical bayes methods for assessing differential expression in microarray experiments. / Stat Appl Genet Mol Biol 2004, 3:Article3.
74. Kussmann M, Raymond F, Affolter M: OMICS-driven biomarker discovery in nutrition and health. / J Biotechnol 2006,124(4):758-87. j.jbiotec.2006.02.014">CrossRef
75. Afman L, Muller M: Nutrigenomics: from molecular nutrition to prevention of disease. / J Am Diet Assoc 2006,106(4):569-76. j.jada.2006.01.001">CrossRef
76. Muller M, Kersten S: Nutrigenomics: goals and strategies. / Nat Rev Genet 2003,4(4):315-22. CrossRef - 作者单位：Heleen M van den Bosch (1) (2)
Meike Bünger (1) (2)
Philip J de Groot (1) (2)
Jolanda van der Meijde (1)
Guido JEJ Hooiveld (1) (2)
Michael Müller (1) (2)
1. Nutrition, Metabolism and Genomics group, Division of Human Nutrition, Wageningen, University, Bomenweg 2, 6703, Wageningen, HD, The Netherlands
2. Nutrigenomics Consortium, TI Food and Nutrition, Wageningen, The Netherlands
文摘
Background Fasting has dramatic effects on small intestinal transport function. However, little is known on expression of intestinal transport and phase I/II metabolism genes during fasting and the role the fatty acid-activated transcription factor PPARα may play herein. We therefore investigated the effects of fasting on expression of these genes using Affymetrix GeneChip MOE430A arrays and quantitative RT-PCR. Results After 24 hours of fasting, expression levels of 33 of the 253 analyzed transporter and phase I/II metabolism genes were changed. Upregulated genes were involved in transport of energy-yielding molecules in processes such as glycogenolysis (G6pt1) and mitochondrial and peroxisomal oxidation of fatty acids (Cact, Mrs3/4, Fatp2, Cyp4a10, Cyp4b1). Other induced genes were responsible for the inactivation of the neurotransmitter serotonin (Sert, Sult1d1, Dtd, Papst2), formation of eicosanoids (Cyp2j6, Cyp4a10, Cyp4b1), or for secretion of cholesterol (Abca1 and Abcg8). Cyp3a11, typically known because of its drug metabolizing capacity, was also increased. Fasting had no pronounced effect on expression of phase II metabolic enzymes, except for glutathione S-transferases which were down-regulated. Time course studies revealed that some genes were acutely regulated, whereas expression of other genes was only affected after prolonged fasting. Finally, we identified 8 genes that were PPARα-dependently upregulated upon fasting. Conclusion We have characterized the response to fasting on expression of transporters and phase I/II metabolic enzymes in murine small intestine. Differentially expressed genes are involved in a variety of processes, which functionally can be summarized as a) increased oxidation of fat and xenobiotics, b) increased cholesterol secretion, c) increased susceptibility to electrophilic stressors, and d) reduced intestinal motility. This knowledge increases our understanding of gut physiology, and may be of relevance for e.g. pre-surgery regimen of patients.