Genome-wide age-related changes in DNA methylation and gene expression in human PBMCs
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- 作者：Wilma T. Steegenga (1)
Mark V. Boekschoten (1)
Carolien Lute (1)
Guido J. Hooiveld (1)
Philip J. de Groot (1)
Tiffany J. Morris (2)
Andrew E. Teschendorff (3)
Lee M. Butcher (2)
Stephan Beck (2)
Michael Müller (1) - 关键词：Molecular aging ; Epigenetics ; DNA methylation ; Gene expression ; PBMCs ; Epigenetic biomarkers of aging
- 刊名：AGE
- 出版年：2014
- 出版时间：June 2014
- 年：2014
- 卷：36
- 期：3
- 全文大小：783 KB
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Mark V. Boekschoten (1)
Carolien Lute (1)
Guido J. Hooiveld (1)
Philip J. de Groot (1)
Tiffany J. Morris (2)
Andrew E. Teschendorff (3)
Lee M. Butcher (2)
Stephan Beck (2)
Michael Müller (1)
1. Division of Human Nutrition, Wageningen University, Bomenweg 2, Wageningen, 6703 HD, The Netherlands
2. Medical Genomics, Paul O’Gorman Building, UCL Cancer Institute, University College London, 72 Huntley Street, London, WC1E 6BT, UK
3. Statistical Cancer Genomics, Paul O’Gorman Building, UCL Cancer Institute, University College London, 72 Huntley Street, London, WC1E 6BT, UK - ISSN：1574-4647
文摘
Aging is a progressive process that results in the accumulation of intra- and extracellular alterations that in turn contribute to a reduction in health. Age-related changes in DNA methylation have been reported before and may be responsible for aging-induced changes in gene expression, although a causal relationship has yet to be shown. Using genome-wide assays, we analyzed age-induced changes in DNA methylation and their effect on gene expression with and without transient induction with the synthetic transcription modulating agent WY14,643. To demonstrate feasibility of the approach, we isolated peripheral blood mononucleated cells (PBMCs) from five young and five old healthy male volunteers and cultured them with or without WY14,643. Infinium 450K BeadChip and Affymetrix Human Gene 1.1 ST expression array analysis revealed significant differential methylation of at least 5?% (ΔYO--?%) at 10,625 CpG sites between young and old subjects, but only a subset of the associated genes were also differentially expressed. Age-related differential methylation of previously reported epigenetic biomarkers of aging including ELOVL2, FHL2, PENK, and KLF14 was confirmed in our study, but these genes did not display an age-related change in gene expression in PBMCs. Bioinformatic analysis revealed that differentially methylated genes that lack an age-related expression change predominantly represent genes involved in carcinogenesis and developmental processes, and expression of most of these genes were silenced in PBMCs. No changes in DNA methylation were found in genes displaying transiently induced changes in gene expression. In conclusion, aging-induced differential methylation often targets developmental genes and occurs mostly without change in gene expression.