Development of SCAR marker for sex identification in dioecious Garcinia gummi-gutta

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• 作者：Kadanthottu Sebastian Joseph (1)
  Hosakatte Niranjana Murthy (1)
  Kundapura Venkataramana Ravishankar (2)
  
• 关键词：DNA markers ; Dioecious ; RAPD ; Sex ; specific fragments ; Tree species
• 刊名：Trees - Structure and Function
• 出版年：2014
• 出版时间：December 2014
• 年：2014
• 卷：28
• 期：6
• 页码：1645-1651
• 全文大小：697 KB
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• 作者单位：Kadanthottu Sebastian Joseph (1)
  Hosakatte Niranjana Murthy (1)
  Kundapura Venkataramana Ravishankar (2)
  
  1. Plant Biotechnology Laboratory, Department of Studies in Botany, Karnatak University, Dharwad, 580003, India
  2. Division of Biotechnology, Indian Institute of Horticultural Research, Hessaraghatta Lake Post, Bangalore, 560089, India
  
• ISSN：1432-2285

文摘

Key message We have developed sex-specific SCAR marker for the identification of dioecious Garcinia gummi - gutta (L.), which is useful for the selection of G. gummi - gutta at seedling stage and for plantation programmes. Abstract Garcinia gummi-gutta (L.) Robs. is a dioecious fruit yielding tree, which is naturally distributed as well as cultivated in the orchards in Western Ghat regions of India. A sex-linked DNA fragment was identified in Garcinia gummi-gutta (L.) Robs. by screening 150 randomly amplified polymorphic DNA primers and only one of them (OPBD20) showed different amplification band pattern associated with sex type. This sex-linked fragment was converted into male-specific sequence-characterized amplified region (SCAR) marker, CAM-566. The primers deigned in this study (OPBD20F and OPBD20R) correctly differentiated 12 male and 12 female plants at high annealing temperatures. Thus, a 556-bp band was amplified in male samples but not in female ones. Nevertheless, it should be noted that the fragments from both sexes were amplified at relatively low annealing temperatures. Additionally, the developed SCAR marker successfully identified the sexes of ten sex-unknown samples. Therefore, it can be used as an effective, convenient and reliable tool for sex determination in such dioecious species.