Surface Marker Epithelial Cell Adhesion Molecule and E-cadherin Facilitate the Identification and Selection of Induced Pluripotent Stem Cells

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• 作者：Hsin-Fu Chen (1) (2)
  Ching-Yu Chuang (4)
  Wen-Chih Lee (3) (8)
  Hsiang-Po Huang (5)
  Han-Chung Wu (3)
  Hong-Nerng Ho (1) (2)
  Yu-Ju Chen (6)
  Hung-Chih Kuo (3) (7) (8) (9)
  
• 关键词：Induced pluripotent stem cells ; EpCAM ; E ; cadherin ; Reprogramming ; Embryonic stem cells
• 刊名：Stem Cell Reviews and Reports
• 出版年：2011
• 出版时间：September 2011
• 年：2011
• 卷：7
• 期：3
• 页码：722-735
• 全文大小：1177KB
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• 作者单位：Hsin-Fu Chen (1) (2)
  Ching-Yu Chuang (4)
  Wen-Chih Lee (3) (8)
  Hsiang-Po Huang (5)
  Han-Chung Wu (3)
  Hong-Nerng Ho (1) (2)
  Yu-Ju Chen (6)
  Hung-Chih Kuo (3) (7) (8) (9)
  
  1. Division of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, College of Medicine and the Hospital, National Taiwan University, Taipei, Taiwan
  2. Graduate Institute of Clinical Genomics, College of Medicine, National Taiwan University, Taipei, Taiwan
  4. Genomics Research Center, Academia Sinica, Taipei, Taiwan
  3. Institute of Cellular and Organismic Biology, Academia Sinica, No. 128, Sec.聽2, Academia Road, Nankang, Taipei, Taiwan
  8. Institute of Biotechnology, National Taiwan Ocean University, Keelung, Taiwan
  5. Division of Medical Research, National Taiwan University Hospital, Taipei, Taiwan
  6. Institute of Chemistry, Academia Sinica, Taipei, Taiwan
  7. Institute of Clinical Medicine, Taipei Medical University, Taipei, Taiwan
  9. Stem Cell Program, Genomics Research Center, Academia Sinica, No. 128, Sec.聽2, Academia Road, Nankang, Taipei, Taiwan
  

文摘

The derivation of induced pluripotent stem cells (iPSCs) requires not only efficient reprogramming methods, but also reliable markers for identification and purification of iPSCs. Here, we demonstrate that surface markers, epithelial cells adhesion molecule (EpCAM) and epithelial cadherin (E-cadherin) can be used for efficient identification and/or isolation of reprogrammed mouse iPSCs. By viral transduction of Oct4, Sox2, Klf4 and n- or c-Myc into mouse embryonic fibroblasts, we observed that the conventional mouse embryonic stem cell (mESC) markers, alkaline phosphatase (AP) and stage-specific embryonic antigen 1 (SSEA1), were expressed in incompletely reprogrammed cells that did not express all the exogenous reprogramming factors or failed to acquire pluripotent status even though exogenous reprogramming factors were expressed. EpCAM and E-cadherin, however, remained inactivated in these cells. Expression of EpCAM and E-cadherin correlated with the activation of Nanog and endogenous Oct4, and was only seen in the successfully reprogrammed iPSCs. Furthermore, purification of EpCAM-expressing cells at late reprogramming stage by FACS enriched the Nanog-expressing cell population suggesting the feasibility of selecting successful reprogrammed mouse iPSCs by EpCAM expression. We have thus identified new surface markers that can efficiently identify successfully reprogrammed iPSCs and provide an effective means for iPSC isolation.