Evaluation of immunomagnetic separation for the improvement of Salmonella detection in aquatic environment

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• 作者：Hsien-Lung Tsai ; Bing-Mu Hsu ; Tsui-Kang Hsu ; Kuan-Hao Huang…
• 关键词：Immunomagnetic separation (IMS) ; Polymerase chain reaction (PCR) ; Salmonella
• 刊名：Environmental Earth Sciences
• 出版年：2015
• 出版时间：June 2015
• 年：2015
• 卷：73
• 期：12
• 页码：7909-7914
• 全文大小：471 KB
• 参考文献：APHA (1995) Standard method for the examination of water and wastewater, 19th edn. APHA, AWWA and WEF, Washington, DC
  Chiu CH, Ou JT (1996) Rapid identification of Salmonella serovars in feces by specific detection of virulence genes, invA and spvC, by an enrichment broth culture-multiplex PCR combination assay. J Clin Microbiol 34(10):2619-622
  Covert TC (1999) Salmonella. In: American Water Works Association Manual of Water Supply Practices, Waterborne Pathogens, AWWA M48. American Water Works Association, Denver, Colorado, Chap 15, pp 107-10
  Cudjoe KS, Krona R (1997) Detection of Salmonella from raw food samples using Dynabeads anti-Salmonella and a conventional reference method. Int J Food Microbiol 137(1):55-2View Article
  Cudjoe KS, Krona R, Olsen E (1994) IMS: a new selective enrichment technique for detection of Salmonella in foods. Int J Food Microbiol 23(2):159-65View Article
  DeCory TR, Durst RA, Zimmerman SJ, Garringer LA, Paluca G, DeCory HH, Montagna RA (2005) Development of an immunomagnetic bead-immunoliposome fluorescence assay for rapid detection of Escherichia coli O157: H7 in aqueous samples and comparison of the assay with a standard microbiological method. Appl Environ Microbiol 71(4):1856-864View Article
  Favrin SJ, Jassim SA, Griffiths MW (2001) Development and optimization of a novel immunomagnetic separation-bacteriophage assay for detection of Salmonella enterica serovar enteritidis in broth. Appl Environ Microbiol 67(1):217-24View Article
  Hardnett FP, Hoekstra RM, Kennedy M, Charles L, Angulo FJ, Emerging Infections Program FoodNet Working Group (2004) Epidemiologic issues in study design and data analysis related to FoodNet activities. Clin Infect Dis 38(Suppl. 3):S121–S126View Article
  Invitrogen (2008) Dynabeads? anti-Salmonella for rapid, selective enrichment of Salmonella. Invitrogen Dynal AS, Oslo
  ISO/DIS (2003) 19250 Water quality-determination of S almonella species (Revision of ISO 6340:1995)-DRAFT. International Organization for Standardization/Draft International Standard
  Kreader CA (1996) Relief of amplification inhibition in PCR with bovine serum albumin or T4 gene 32 protein. Appl Environ Microbiol 62(3):1102-106
  Mansfield LP, Forsythe SJ (1993) Immunomagnetic separation as an alternative to enrichment broths for Salmonella detection. Lett Appl Microbiol 16(3):122-25View Article
  Mercanoglu B, Griffiths MW (2005) Combination of immunomagnetic separation with real-time PCR for rapid detection of Salmonella in milk, ground beef, and alfalfa sprouts. J Food Prot 68(3):557-61
  Notzon A, Helmuth R, Bauer J (2006) Evaluation of an immunomagnetic separation real-time PCR assay for the rapid detection of Salmonella in meat. J Food Prot 69(12):2896-901
  Rossen L, Norskov P, Holmstrom K, Rasmussen OF (1992) Inhibition of PCR by components of food samples, microbial diagnostic assays and DNA-extraction solutions. Int J Food Microbiol 17(1):37-5View Article
  Sano D, Fukushi K, Yoshida Y, Omura T (2003) Detection of enteric viruses in municipal sewage sludge by a combination of the enzymatic virus elution method and RT-PCR. Water Res 37(14):3490-498View Article
  Skjerve E, Olsvik O (1991) Immunomagnetic separation of Salmonella from foods. Int J Food Microbiol 14:11-7View Article
  Taban BM, Ben U, Aytac SA (2009) Rapid detection of Salmonella in milk by combined immunomagnetic separation-polymerase chain reaction assay. J Dairy Sci 92(6):2382-388View Article
  Voetsch AC, Van Gilder TJ, Angulo FJ, Farley MM, Shallow S, Marcus R, Cieslak PR, Deneen VC, Tauxe RV, Emerging Infections Program FoodNet Working Group (2004) FoodNet estimate of the burden of illness caused by nontyphoidal Salmonella infections in the United States. Clin Infect Dis 38(Suppl 3):S127–S134View Article
  Wilson IG (1997) Inhibition and facilitation of nucleic acid amplification. Appl Environ Microbiol 63(10):3741-751
  
• 作者单位：Hsien-Lung Tsai (1)
  Bing-Mu Hsu (2)
  Tsui-Kang Hsu (3) (4)
  Kuan-Hao Huang (4)
  Feng-Cheng Shih (5)
  Jung-Sheng Chen (6)
  Hung-Jen Wang (2)
  Po-Min Kao (2)
  Hung-Chang Su (7)
  
  1. Department of Emergency Medicine, Cheng Hsin General Hospital, Taipei, Taiwan, ROC
  2. Department of Earth and Environmental Sciences, National Chung Cheng University, Chiayi, Taiwan, ROC
  3. Department of Ophthalmology, Cheng Hsin General Hospital, Taipei, Taiwan, ROC
  4. Department of Life Science, Institute of Molecular Biology, Institute of Biomedical Science, National Chung Cheng University, Chiayi, Taiwan, ROC
  5. Department of Environmental Engineering and Health, Yuanpei University, Hsinchu, Taiwan, ROC
  6. Graduate Institute of Life Sciences, National Defense Medical Center, Taipei, Taiwan, ROC
  7. Department of Anesthesiology, Surgical Department, Cheng Hsin General Hospital, Taipei, Taiwan, ROC
  
• 刊物类别：Earth and Environmental Science
• 刊物主题：None Assigned
• 出版者：Springer Berlin Heidelberg
• ISSN：1866-6299

文摘

This study compares various immunomagnetic separation (IMS) protocols. The average recovery efficiency of IMS for different Salmonella concentrations (between 102 and 105 CFU/ml) was 53.5?±?12.9?% (n?=?24). When turbidity was less than 1,000 NTU, altering the debris ratio of water samples produced no significant changes in the recovery efficiency of IMS. Prolonging the incubation time or increasing the amount of immunomagnetic beads increased the recovery efficiency of IMS. This study also compares processes in which water samples were filtered, eluted/concentrated, and then processed by the IMS and non-IMS methods before PCR. Results indicate that IMS can effectively eliminate these inhibitors prior to polymerase chain reaction (PCR). The detection limit of Salmonella analysis process with IMS separation before enrichment was superior to IMS separation after enrichment. The proposed Salmonella analysis process, which included IMS to concentrate and purify the Salmonella, improves the sensitivity and the detection efficiency of the entire analysis process.