Urinary metabolites of di(2-ethylhexyl) phthalate relation to sperm motility, reactive oxygen species generation, and apoptosis in polyvinyl chloride workers
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- 作者：Li-Ping Huang (1) (2)
Ching-Chang Lee (3)
Jer-Pei Fan (3)
Po-Hsiu Kuo (4)
Tung-Sheng Shih (5) (6)
Ping-Chi Hsu (1) - 关键词：Di(2 ; ethylhexyl) phthalate ; Urinary metabolites ; Semen quality ; Apoptosis ; TUNEL assay
- 刊名：International Archives of Occupational and Environmental Health
- 出版年：2014
- 出版时间：August 2014
- 年：2014
- 卷：87
- 期：6
- 页码：635-646
- 全文大小：272 KB
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43. World Health Organization (2010) WHO laboratory manual for the examination and processing of human semen, 5th edn. WHO Press, Geneva - 作者单位：Li-Ping Huang (1) (2)
Ching-Chang Lee (3)
Jer-Pei Fan (3)
Po-Hsiu Kuo (4)
Tung-Sheng Shih (5) (6)
Ping-Chi Hsu (1)
1. Department of Safety, Health and Environmental Engineering, National Kaohsiung First University of Science and Technology, No. 2, Jhuoyue Rd., Nanzih District, Kaohsiung City, 811, Taiwan
2. Department of Nursing, Chung-Jen College of Nursing, Health Sciences and Management, Chiayi City, Taiwan
3. Department of Environmental and Occupational Health, National Cheng Kung University, Tainan City, Taiwan
4. Institute of Epidemiology and Preventive Medicine, College of Public Health, National Taiwan University, Taipei City, Taiwan
5. Institute of Occupational Safety and Health, Council of Labor Affairs, Taipei City, Taiwan
6. Graduate Institute of Environmental Health, College of Public Health, China Medical University, Taichung City, Taiwan - ISSN：1432-1246
文摘
Purpose To measure the concentrations of urinary di(2-ethylhexyl) phthalate (DEHP) metabolites in polyvinyl chloride (PVC) workers and a control group for determining the relationship of DEHP exposure to semen quality, sperm reactive oxygen species (ROS) generation, and sperm apoptosis. Methods We assessed the metabolites of DEHP, namely urinary mono-(2-ethylhexyl) phthalate (MEHP), mono-(2-ethyl-5-hydroxyhexyl) phthalate (MEHHP), and mono-(2-ethyl-5-oxohexyl) phthalate (MEOHP), and semen quality, such as sperm concentration, motility, morphology, ROS generation, and DNA damage by using terminal deoxynucleotidyl transferase-mediated nick end labeling assay obtained from 47 workers employed within two PVC pellet plants and 15 graduate students in Taiwan. Results Sperm concentration and motility were significantly affected in the high-exposure group. The percentage and intensity of sperm ROS generation were higher in the high-exposure group than those in the control group. After adjustment for age, smoking status, and coffee consumption, the decrease in sperm motility was inversely associated with the concentration of MEHP (β?=??.549, p?=?0.0085), MEHHP (β?=??.155, p?=?0.0074), and MEOHP (β?=??.201, p?=?0.0041). Moreover, sperm apoptosis and ROS generation were positively associated with MEHHP and MEOHP concentration, respectively. Conclusions This was the first study to explore the associations between levels of DEHP exposure, sperm motility, ROS generation, and apoptosis. The results suggested that urinary MEHHP and MEOHP were sensitive biomarkers for reflecting the relationship between DEHP exposure and semen quality.