Visualizing the replicating HSV-1 virus using STED super-resolution microscopy
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- 作者：Zhuoran Li ; Ce Fang ; Yuanyuan Su ; Hongmei Liu ; Fengchao Lang ; Xin Li…
- 关键词：HSV ; 1 replication ; STED ; IF ; FISH ; RNA Pol II ; ICP8
- 刊名：Virology Journal
- 出版年：2016
- 出版时间：December 2016
- 年：2016
- 卷：13
- 期：1
- 全文大小：1,811 KB
- 刊物主题：Virology;
- 出版者：BioMed Central
- ISSN：1743-422X
文摘
Background Replication of viral genome is the central event during the lytic infectious cycle of herpes simplex virus 1 (HSV-1). However, the details of HSV-1 replication process are still elusive due to the limitations of current molecular and conventional fluorescent microscopy methods. Stimulated emission depletion (STED) microscopy is one of the recently available super-resolution techniques allowing observation at sub-diffraction resolution.