Identification and characterization of HTD2: a novel gene negatively regulating tiller bud outgrowth in rice
详细信息 查看全文
- 作者：Wenzhen Liu (1)
Chao Wu (1)
Yaping Fu (1)
Guocheng Hu (1)
Huamin Si (1)
Li Zhu (1)
Weijiang Luan (1)
Zhengquan He (1) (2)
Zongxiu Sun (1) - 关键词：HTD2 ; High tillering ; Dwarf ; Map ; based cloning ; T ; DNA ; Rice (Oryza sativa L.)
- 刊名：Planta
- 出版年：2009
- 出版时间：September 2009
- 年：2009
- 卷：230
- 期：4
- 页码：649-658
- 全文大小：595KB
- 参考文献：1. Arite T, Iwata H, Ohshima K, Maekawa M, Nakajima M, Kojima M, Sakakibara H, Kyozuka J (2007) / DWARF10, an / RMS1/MAX4/DAD1 ortholog, controls lateral bud outgrowth in rice. Plant J 51:1019-029 CrossRef
2. Bennett T, Sieberer T, Willett B, Booker J, Luschnig C, Leyser O (2006) The / Arabidopsis MAX pathway controls shoot branching by regulating auxin transport. Curr Biol 16:553-63 CrossRef
3. Beveridge CA (2000) Long-distance signalling and a mutational analysis of branching in pea. Plant Growth Regul 32:193-03 CrossRef
4. Beveridge CA (2006) Axillary bud outgrowth: sending a message. Curr Opin Plant Biol 9:35-0 CrossRef
5. Beveridge CA, Symons GM, Murfet IC, Ross JJ, Rameau C (1997) The / rms1 mutant of pea has elevated indole-3-acetic acid levels and reduced root-sap zeatin riboside content but increased branching controlled by graft-transmissible signals. Plant Physiol 115:1251-258
6. Booker J, Auldridge M, Wills S, McCarty D, Klee H, Leyser O (2004) MAX3/CCD7 is a carotenoid cleavage dioxygenase required for the synthesis of a novel plant signaling molecule. Curr Biol 14:1232-238 CrossRef
7. Booker J, Sieberer T, Wright W, Williamson L, Willett B, Stirnberg P, Turnbull C, Srinivasan M, Goddard P, Leyser O (2005) / MAX1 encodes a cytochrome P450 family member that acts downstream of / MAX3/4 to produce a carotenoid-derived branch-inhibiting hormone. Dev Cell 8:443-49 CrossRef
8. Brody MS, Vijay K, Price CW (2001) Catalytic function of an alpha/beta hydrolase is required for energy stress activation of the sigma(B) transcription factor in / Bacillus subtilis. J Bacteriol 183:6422-428 CrossRef
9. Cheng Y, Dai X, Zhao Y (2006) Auxin biosynthesis by the YUCCA flavin monooxygenases controls the formation of floral organs and vascular tissues in / Arabidopsis. Genes Dev 20:1790-799 CrossRef
10. Emanuelsson O, Nielsen H, Brunak S, von Heijne G (2000) Predicting subcellular localization of proteins based on their N-terminal amino acid sequence. J Mol Biol 300:1005-016 CrossRef
11. Gomez-Roldan V, Fermas S, Brewer PB, Puech-Pages V, Dun EA, Pillot JP, Letisse F, Matusova R, Danoun S, Portais JC, Bouwmeester H, Becard G, Beveridge CA, Rameau C, Rochange SF (2008) Strigolactone inhibition of shoot branching. Nature 455:189-94 CrossRef
12. Ishikawa S, Maekawa M, Arite T, Onishi K, Takamure I, Kyozuka J (2005) Suppression of tiller bud activity in tillering dwarf mutants of rice. Plant Cell Physiol 46:79-6 CrossRef
13. Kim SR, Lee J, Jun SH, Park S, Kang HG, Kwon S, An G (2003) Transgene structures in T-DNA-inserted rice plants. Plant Mol Biol 52:761-73 CrossRef
14. Li X, Qian Q, Fu Z, Wang Y, Xiong G, Zeng D, Wang X, Liu X, Teng S, Hiroshi F, Yuan M, Luo D, Han B, Li J (2003) Control of tillering in rice. Nature 422:618-21 CrossRef
15. Liu Y, Mitsukawa N, Oosumi T, Whittier RF (1995) Efficient isolation and mapping of / Arabidopsis thaliana T-DNA insert junctions by thermal asymmetric interlaced PCR. Plant J 8:457-63 CrossRef
16. Liu W, Fu Y, Hu G, Si H, Zhu L, Wu C, Sun Z (2007) Identification and fine mapping of a thermo-sensitive chlorophyll-deficient mutant in rice ( / Oryza sativa L.). Planta 226:785-95 CrossRef
17. Ljung K, Bhalerao RP, Sandberg G (2001) Sites and homeostatic control of auxin biosynthesis in / Arabidopsis during vegetative growth. Plant J 28:465-74 CrossRef
18. McCouch SR, Teytelman L, Xu YB, Lobos KB, Clare K, Walton M, Fu B, Maghirang R, Li Z, Xing Y, Zhang Q, Kono I, Yano M, Fjellstrom R, DeClerck G, Schneider D, Cartinhour S, Ware D, Stein L (2002) Development and mapping of 2240 new SSR markers for rice ( / Oryza sativa L.). DNA Res 9:199-07 CrossRef
19. McSteen P, Leyser O (2005) Shoot branching. Annu Rev Plant Biol 56:353-74 CrossRef
20. Murray MG, Thompson WF (1980) Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res 8:4321-325 CrossRef
21. Napoli C (1996) Highly branched phenotype of the petunia dad1- mutant is reversed by grafting. Plant Physiol 111:27-7
22. Napoli CA, Ruehle J (1996) New mutations affecting meristem growth and potential in / Petunia hybrida Vilm. J Hered 87:371-77
23. Ongaro V, Leyser O (2008) Hormonal control of shoot branching. J Exp Bot 59:67-4 CrossRef
24. Rameau C, Murfet IC, Laucou V, Floyd RS, Morris SE, Beveridge CA (2002) Pea / rms6 mutants exhibit increased basal branching. Physiol Plant 115:458-67 CrossRef
25. Ryu CH, You JH, Kang HG, Hur J, Kim YH, Han MJ, An K, Chung BC, Lee CH, An G (2004) Generation of T-DNA tagging lines with a bidirectional gene trap vector and the establishment of an insertion-site database. Plant Mol Biol 54:489-02 CrossRef
26. Schwartz SH, Qin X, Loewen MC (2004) The biochemical characterization of two carotenoid cleavage enzymes from / Arabidopsis indicates that a carotenoid-derived compound inhibits lateral branching. J Biol Chem 279:46940-6945 CrossRef
27. Simons JL, Napoli CA, Janssen BJ, Plummer KM, Snowden KC (2007) Analysis of the / DECREASED APICAL DOMINANCE genes of petunia in the control of axillary branching. Plant Physiol 143:697-06 CrossRef
28. Sorefan K, Booker J, Haurogne K, Goussot M, Bainbridge K, Foo E, Chatfield S, Ward S, Beveridge C, Rameau C, Leyser O (2003) / MAX4 and / RMS1 are orthologous dioxygenase-like genes that regulate shoot branching in / Arabidopsis and pea. Genes Dev 17:1469-474 CrossRef
29. Stirnberg P, Chatfield SP, Leyser HM (1999) / AXR1 acts after lateral bud formation to inhibit lateral bud growth in / Arabidopsis. Plant Physiol 121:839-47 CrossRef
30. Stirnberg P, van De Sande K, Leyser HM (2002) / MAX1 and / MAX2 control shoot lateral branching in / Arabidopsis. Development 129:1131-141
31. Stirnberg P, Furner IJ, Ottoline Leyser HM (2007) / MAX2 participates in an SCF complex which acts locally at the node to suppress shoot branching. Plant J 50:80-4 CrossRef
32. Takeda T, Suwa Y, Suzuki M, Kitano H, Ueguchi-Tanaka M, Ashikari M, Matsuoka M, Ueguchi C (2003) The / OsTB1 gene negatively regulates lateral branching in rice. Plant J 33:513-20 CrossRef
33. Tamura K, Dudley J, Nei M, Kumar S (2007) MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol Biol Evol 24:1596-599 CrossRef
34. Tanaka M, Takei K, Kojima M, Sakakibara H, Mori H (2006) Auxin controls local cytokinin biosynthesis in the nodal stem in apical dominance. Plant J 45:1028-036 CrossRef
35. Temnykh S, DeClerck G, Lukashova A, Lipovich L, Cartinhour S, McCouch S (2001) Computational and experimental analysis of microsatellites in rice ( / Oryza sativa L.): frequency, length, variation, transposon associations, and genetic marker potential. Genome Res 11:1441-452 CrossRef
36. Umehara M, Hanada A, Yoshida S, Akiyama K, Arite T, Takeda-Kamiya N, Magome H, Kamiya Y, Shirasu K, Yoneyama K, Kyozuka J, Yamaguchi S (2008) Inhibition of shoot branching by new terpenoid plant hormones. Nature 455:195-00 CrossRef
37. Vain P, Thole V (2009) Gene insertion patterns and sites. Methods Mol Biol 478:203-26 CrossRef
38. Wang MB, Waterhouse PM (1997) A rapid and simple method of assaying plants transformed with hygromycin or PPT resistance genes. Plant Mol Biol Rep 15:209-15 CrossRef
39. Ward SP, Leyser O (2004) Shoot branching. Curr Opin Plant Biol 7:73-8 CrossRef
40. Xu M, Zhu L, Shou H, Wu P (2005) A PIN1 family gene, / OsPIN1, involved in auxin-dependent adventitious root emergence and tillering in rice. Plant Cell Physiol 46:1674-681 CrossRef
41. Zhu ZG, Xiao H, Fu YP, Hu GC, Yu YH, Si HM, Zhang JL, Sun ZX (2001) Construction of transgenic rice populations by inserting the maize transponson Ac/Ds and genetic analysis for several mutants. Chin J Biotech 17:288-92 (in Chinese with English abstract)
42. Zou J, Zhang S, Zhang W, Li G, Chen Z, Zhai W, Zhao X, Pan X, Xie Q, Zhu L (2006) The rice / HIGH-TILLERING DWARF1 encoding an ortholog of / Arabidopsis MAX3 is required for negative regulation of the outgrowth of axillary buds. Plant J 48:687-98 CrossRef - 作者单位：Wenzhen Liu (1)
Chao Wu (1)
Yaping Fu (1)
Guocheng Hu (1)
Huamin Si (1)
Li Zhu (1)
Weijiang Luan (1)
Zhengquan He (1) (2)
Zongxiu Sun (1)
1. State Key Laboratory of Rice Biology, China National Rice Research Institute, 359 Tiyuchang Road, 310006, Hangzhou, Zhejiang, China
2. Biotechnology Research Center, China Three Gorges University, 443002, Yichang, Hubei, China
文摘
Tiller number is highly regulated by controlling the formation of tiller bud and its subsequent outgrowth in response to endogenous and environmental signals. Here, we identified a rice mutant htd2 from one of the 15,000 transgenic rice lines, which is characterized by a high tillering and dwarf phenotype. Phenotypic analysis of the mutant showed that the mutation did not affect formation of tiller bud, but promoted the subsequent outgrowth of tiller bud. To isolate the htd2 gene, a map-based cloning strategy was employed and 17 new insertions-deletions (InDels) markers were developed. A high-resolution physical map of the chromosomal region around the htd2 gene was made using the F2 and F3 population. Finally, the gene was mapped in 12.8?kb region between marker HT41 and marker HT52 within the BAC clone OSJNBa0009J13. Cloning and sequencing of the target region from the mutant showed that the T-DNA insertion caused a 463?bp deletion between the promoter and first exon of an esterase/lipase/thioesterase family gene in the 12.8?kb region. Furthermore, transgenic rice with reduced expression level of the gene exhibited an enhanced tillering and dwarf phenotype. Accordingly, the esterase/lipase/thioesterase family gene (TIGR locus Os03g10620) was identified as the HTD2 gene. HTD2 transcripts were expressed mainly in leaf. Loss of function of HTD2 resulted in a significantly increased expression of HTD1, D10 and D3, which were involved in the strigolactone biosynthetic pathway. The results suggest that the HTD2 gene could negatively regulate tiller bud outgrowth by the strigolactone pathway.