On-Line HPLC with Biochemical Detection for Screening Bioactive Compounds in Complex Matrixes

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• 作者：Wen-Bin Peng ; Jing-Ling Tan ; Dan-Dan Huang ; Xiao-Ping Ding
• 关键词：High ; performance liquid chromatography (HPLC) ; Mass spectrometry (MS) ; Nuclear magnetic resonance (NMR) ; Biochemical assay ; Free radicals ; Enzyme ; Receptor
• 刊名：Chromatographia
• 出版年：2015
• 出版时间：December 2015
• 年：2015
• 卷：78
• 期：23-24
• 页码：1443-1457
• 全文大小：1,232 KB
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• 作者单位：Wen-Bin Peng (1)
  Jing-Ling Tan (1)
  Dan-Dan Huang (1)
  Xiao-Ping Ding (1)
  
  1. Hubei Institution of Traditional Chinese Medicine Control, Hubei Institution for Food and Drug Control, Dingziqiaolu 54#, Wuhan, 430064, China
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Analytical Chemistry
  Organic Chemistry
  Pharmacy
  Biochemistry
  Plant Sciences
  Measurement Science and Instrumentation
  
• 出版者：Vieweg Verlag
• ISSN：1612-1112

文摘

On-line high-performance liquid chromatography (HPLC) coupled with biochemical detection (BCD) has been developed to screen compounds showing antioxidant action, enzyme inhibition and receptor affinity in complex matrixes. This review summarizes HPLC methods combining different post-column detection methods, such as diode-array detection (DAD), mass spectrometry (MS), chemiluminescence (CL) and nuclear magnetic resonance, for antioxidant screening. The methods based on a single relatively stable reagent such as DPPH-/sup> and ABTS- were the most popular. Oxygen free radical scavengers mainly depended on post-column CL detection. The on-line hyphenated HPLC–BCD systems based on post-column UV/DAD fluorescence and MS detection were also widely applied to screen enzyme- and receptor-active compounds. These strategies provide a convenient tool for quick identification and quantification of active compounds in complex matrixes. Keywords High-performance liquid chromatography (HPLC) Mass spectrometry (MS) Nuclear magnetic resonance (NMR) Biochemical assay Free radicals Enzyme Receptor