Determination of Pb genotoxic effects in Allium cepa root cells by fluorescent probe, microtubular immunofluorescence and comet assay

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• 作者：Ze Jiang (1)
  Rong Qin (1) (2)
  Huanhuan Zhang (1)
  Jinhua Zou (1)
  Qiuyue Shi (1)
  Junran Wang (1)
  Wusheng Jiang (1)
  Donghua Liu (1)
  
• 关键词：Lead (Pb) ; Allium cepa var. agrogarum ; Microtubule (MT) ; Immunofluorescent microscopy ; Comet assay
• 刊名：Plant and Soil
• 出版年：2014
• 出版时间：October 2014
• 年：2014
• 卷：383
• 期：1-2
• 页码：357-372
• 全文大小：1,603 KB
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• 作者单位：Ze Jiang (1)
  Rong Qin (1) (2)
  Huanhuan Zhang (1)
  Jinhua Zou (1)
  Qiuyue Shi (1)
  Junran Wang (1)
  Wusheng Jiang (1)
  Donghua Liu (1)
  
  1. Tianjin Key Laboratory of Animal and Plant Resistance, College of Life Sciences, Tianjin Normal University, Tianjin, 300387, People鈥檚 Republic of China
  2. School of Life Science, South China Normal University, Guangzhou, 510631, People鈥檚 Republic of China
  
• ISSN：1573-5036

文摘

Aims In order to further understand the toxic effects of Pb on root tip cells of Allium cepa var. agrogarum, root growth, Pb uptake, cell division, DNA damage and MT cytoskeleton were investigated. Methods Fluorescence labeling; Carbol Fuchsin-staining; indirect immunofluorescent microscopy; comet assay. Results Pb ions entered A. cepa root meristem cells within hours of exposure. The mitotic index (MI) decreased progressively with increasing of Pb concentration. Chromosomal segregation and motility are inhibited, which is directly correlated with the disorganization of the corresponding MT arrays. Pb may interact with tubulin and hinder the polymerization process, resulting in defective mitotic spindles. Pb induced DNA damage in A. cepa root nuclei. Conclusion Root tips of plants are the most sensitive organ to environmental stresses. Root apical meristems play a key role in the immediate reaction to stress factors by activating signal cascades to the other plant organs. The data presented above can be used as valuable and early markers in cellular changes induced by metals for the evaluation of metal contamination and can be used as early warning signals for general or particular stress.