Amperometric nitrite biosensor based on a gold electrode modified with cytochrome c on Nafion and Cu-Mg-Al layered double hydroxides

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• 作者：Huanshun Yin ; Yunlei Zhou ; Tao Liu ; Lin Cui ; Shiyun Ai ; Yanyan Qiu and Lusheng Zhu
• 关键词：Cu ; Mg ; Al layered double hydroxide ; Nafion ; Cytochrome c ; Nitrite biosensor ; Direct electrochemistry ; Determination
• 刊名：Microchimica Acta
• 出版年：2010
• 出版时间：December 2010
• 年：2010
• 卷：171
• 期：3-4
• 页码：385-392
• 全文大小：269.6 KB

文摘

An amperometric biosensor for nitrite was prepared by immobilizing cytochrome c (Cyt c) on a gold electrode that was modified with Nafion and a Cu-Mg-Al layered double hydroxide (Cu-LDH). The Cu-LDH was characterized by Fourier transform infrared spectroscopy and powder X-ray diffraction. The UV-visible spectrum suggests that Cyt c retains its native conformation in the modified film. The direct electrochemical investigation indicated that the composite film represents a good platform for the immobilization of Cyt c as well as an excellent promoter for the electron transfer between Cyt c and the gold electrode. Moreover, the biosensor showed a remarkable bioelectrocatalytic activity for the oxidation of nitrite with a linear range from 0.75 to 123 μM. The detection limit is 2 ¡Á 10−7 M (S/N = 3). The biosensor was successfully applied to the determination of nitrite in food samples.