Changes in the membrane fatty acid composition in Anoxybacillus flavithermus subsp. yunnanensis E13T as response to solvent stress
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- 作者：Hui Peng ; Lu Yi ; Xiaohan Zhang ; Yazhong Xiao ; Yi Gao ; Chao He
- 关键词：Anoxybacillus flavithermus ssp. yunnanesis ; Organic solvent ; tolerant ; Straight ; chain fatty acid ; High temperature ; Stress response
- 刊名：Archives of Microbiology
- 出版年：2017
- 出版时间：January 2017
- 年：2017
- 卷：199
- 期：1
- 页码：1-8
- 全文大小：
- 刊物类别：Biomedical and Life Sciences
- 刊物主题：Microbiology; Microbial Ecology; Biochemistry, general; Cell Biology; Biotechnology; Ecology;
- 出版者：Springer Berlin Heidelberg
- ISSN：1432-072X
- 卷排序：199
文摘
Anoxybacillus flavithermus subsp. yunnanensis is currently the first species of strictly thermophilic bacteria that is able to tolerate a broad range of solvents. Unlike most of solvent-tolerant mesophilic bacteria, the bacterium does not synthesize unsaturated fatty acids. Our results revealed that in growing cells of A. flavithermus subsp. yunnanensis E13T, ethanol and toluene resulted in an increase in straight-chain fatty acids, mainly C16:0, leading to a more rigid membrane. Moreover, the increase in straight-chain fatty acids caused by ethanol was much higher than that of toluene. High temperature had little effect on the fatty acid composition by itself, whereas the combined conditions of high temperature and ethanol caused the dramatic increase in straight-chain fatty acids (mainly C16:0), that was balanced by decreasing branched fatty acids. The increase was also temperature dependent. The proportion of C16:0 further increased above 60 °C. No similar evidence was found in four other species of Anoxybacillus. The results suggested that A. flavithermus subsp. yunnanesis seems to develop a different response to solvents compared to its mesophilic counterparts, which consist of an increase in the saturated straight/branched ratio.