Over-expression of genes and proteins of ubiquitin specific peptidases (USPs) and proteasome subunits (PSs) in breast cancer tissue observed by the methods of RFDD-PCR and proteomics

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• 作者：Shishan Deng ; Hongying Zhou ; Ruohong Xiong ; Youguang Lu ; Dazhong Yan ; Tianyong Xing ; Lihua Dong ; Enjie Tang and Huijun Yang
• 关键词：Breast cancer ; 2 ; DE ; Mass spectrometry ; Proteasome subuinits ; RFDD ; PCR ; Ubiquitin specific proteases
• 刊名：Breast Cancer Research and Treatment
• 出版年：2007
• 出版时间：July, 2007
• 年：2007
• 卷：104
• 期：1
• 页码：21-30
• 全文大小：324 KB
• 刊物类别：Medicine
• 刊物主题：Medicine & Public Health
  Oncology
  
• 出版者：Springer Netherlands
• ISSN：1573-7217

文摘

The ubiquitin–proteasome system facilitates the degradation of damaged proteins and regulators of growth and stress response. Alterations in this proteolytic system are associated with a variety of human pathologies. By restriction fragment differential display polymerase chain reaction (RFDD-PCR) and matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometry (MALDI-TOF-TOF MS) based on two-dimensional polyacrylamide gel electrophoresis (2-DE), differentially expressed genes and proteins of ubiquitin specific proteases (USPs), proteasome subuinits (PSs) and ubiquitin protein ligase E3A (UBE3A) were analyzed between breast cancer and adjacent normal tissues. Some of them were further verified as over-expression by immunohistochemical stain. Five genes of proteasome subunits (PSs), including PSMB5, PSMD1, PSMD2, PSMD8 and PSMD11, four genes of USPs, including USP9X, USP9Y, USP10 and USP25, and ubiquitin protein ligase E3A (UBE3A) were over-expressed (>3-fold) in breast cancer tissue compared to adjacent normal tissue, and over-expression (>4-fold) of proteins of PSMA1 and SMT3A were observed in breast cancer tissue. PSMD8, PSMD11 and UBE3A were further verified as over-expression by immunohistochemical stain. The action of ubiquitin–proteasome system were obviously enhanced in breast cancer, and selectively intervention in action of ubiquitin–proteasome system may be a useful method of treating human breast cancer.