Fluorescence polarization immunoassay using IgY antibodies for detection of valnemulin in swine tissue

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• 作者：Huiyan Zhang ; Tiejun Mi ; Oleg Yu Khan…
• 关键词：Fluorescence polarization immunoassay ; IgY ; Chemical residues ; Swine tissue ; Valnemulin
• 刊名：Analytical and Bioanalytical Chemistry
• 出版年：2015
• 出版时间：October 2015
• 年：2015
• 卷：407
• 期：25
• 页码：7843-7848
• 全文大小：1,622 KB
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  5.Lippolis V, Pascale M, Valenzano S, Pluchinotta V, Baumgartner S, Krska R, Visconti A (2011) A rapid fluorescence polarization immunoassay for the determination of T-2 and HT-2 toxins in wheat. Anal Bioanal Chem 401(8):2561-571CrossRef
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• 作者单位：Huiyan Zhang (1)
  Tiejun Mi (1)
  Oleg Yu Khan (2)
  Yajie Sheng (1)
  Sergei A. Eremin (2)
  Ross C. Beier (3)
  Suxia Zhang (1)
  Jianzhong Shen (1)
  Zhanhui Wang (1)
  
  1. College of Veterinary Medicine, China Agricultural University, State Key Laboratory of Agrobiotechnology, Beijing Laboratory for Food Quality and Safety, Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety, Beijing, 100193, China
  2. Department of Chemical Enzymology, Faculty of Chemistry, M. V. Lomonosov Moscow State University, Moscow, 119992, Russia
  3. U.S. Department of Agriculture, Agricultural Research Service, Southern Plains Agricultural Research Center, Food and Feed Safety Research Unit, College Station, TX, 77845, USA
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Analytical Chemistry
  Food Science
  Inorganic Chemistry
  Physical Chemistry
  Monitoring, Environmental Analysis and Environmental Ecotoxicology
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1618-2650

文摘

Immunoglobulin Y (IgY) is derived from egg yolk and has been identified as a cheap and high-yield immunoreagent. The application of IgY in immunoassays for the detection of chemical contaminants in food samples has rarely been reported. In this work, we describe a rapid and sensitive fluorescence polarization immunoassay (FPIA) for valnemulin (VAL) using IgY which was produced using a previously prepared immunogen. Three fluorophore-labeled VAL tracers were synthesized and the sensitivity of the best tracer (VAL–DTAF) in the optimized FPIA with antibody IgY₁₀₀ demonstrated an IC₅₀ value of 12 ng mL? in buffer. After evaluation of several extraction procedures, acidified acetonitrile was selected to extract VAL from swine tissue. The recoveries of VAL in spiked swine tissue at three levels (50, 100, and 200 μg kg?) were higher than 79 % with coefficients of variation (CVs) lower than 12 %. The limit of detection (LOD) of the FPIA in swine tissue was 26 μg kg? and was lower than the maximum residue limit (MRL) of VAL set by the European Union. The study showed that IgY could be a good substitute for IgG when developing a high-throughput assay for chemical residues.