Field evaluation and fiber analysis of transgenic cotton
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- 作者：Bushra Rashid (1)
Ghazanfar Ali Khan (1)
Tayyab Husnain (1)
Sheikh Riazuddin (1) - 关键词：cry1Ac ; cry2A ; insect resistance ; dual Bt cotton ; field studies ; fiber
- 刊名：Journal of Crop Science and Biotechnology
- 出版年：2009
- 出版时间：September 2009
- 年：2009
- 卷：12
- 期：3
- 页码：135-141
- 全文大小：727KB
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Ghazanfar Ali Khan (1)
Tayyab Husnain (1)
Sheikh Riazuddin (1)
1. National Centre of Excellence in Molecular Biology, 87-W.Canal Bank Road Thokar Niaz Baig, Lahore, 53700, Pakistan
文摘
We report the field evaluation of second generation of transgenic cotton expressing Bacillus thuringiensis (Bt) genes cry1Ac and cry2A under CaMV 35S promoter. Sixty-five transgenic lines were grown under RCBD design. Transgenic plants exhibited inherent ability to resist target insect (p < 0.05 and 0.01). Morphological studies showed significant reduction in plant height making them favorable for breeding. Yield was significantly increased for the transgenic lines. Fiber analysis showed improved gin turn out 40% for transgenic lines in comparison to 32% for non-transformed lines. Fibre quality of transgenic lines was not affected when compared with non transgenic lines. Inheritance pattern for transgenic lines suggests the need of further studies to understand the complex molecular mechanisms for resistance management and biosafety studies to develop new Bt cotton varieties.