Immunological characterization of monoclonal antibodies used in rapid influenza diagnostic test for detection of the 2009 pandemic influenza A(H1N1)pdm09 infection

详细信息    查看全文

• 作者：Hwajung Yi ; Mi-Seon Lee ; Joo-Yeon Lee ; Hae Kyung Lee ; Chun Kang
• 关键词：2009 influenza pandemic ; monoclonal antibody ; RIDT ; H1N1pdm09 ; 1918 H1N1
• 刊名：Journal of Microbiology
• 出版年：2015
• 出版时间：February 2015
• 年：2015
• 卷：53
• 期：2
• 页码：166-175
• 全文大小：1,068 KB
• 参考文献：1. Amano, Y. and Cheng, Q. 2005. Detection of influenza virus: traditional approaches and development of biosensors. / Anal. Bioanal. Chem. 381, 156-64. CrossRef
  2. Brockwell-Staats, C., Webster, R.G., and Webby, R.J. 2009. Diversity of influenza viruses in swine and the emergence of a novel human pandemic influenza A (H1N1). / Influenza Other Respir. Viruses 3, 207-13. CrossRef
  3. Carrat, F. and Flahault, A. 2007. Influenza vaccine: the challenge of antigenic drift. / Vaccine 25, 6852-862. CrossRef
  4. Choi, Y.J., Kim, H.J., Park, J.S., Oh, M.H., Nam, H.S., Kim, Y.B., Cho, B.K., Ji, M.J., and Oh, J.S. 2010. Evaluation of new rapid antigen test for detection of pandemic influenza A/H1N1 2009 virus. / J. Clin. Microbiol. 48, 2260-262. CrossRef
  5. Corti, D., Suguitan, A.L. Jr., Pinna, D., Silacci, C., Fernandez-Rodriguez, B.M., Vanzetta, F., Santos, C., Luke, C.J., Torres-Velez, F.J., and Temperton, N.J. 2010. Heterosubtypic neutralizing antibodies are produced by individuals immunized with a seasonal influenza vaccine. / J. Clin. Invest. 120, 1663. CrossRef
  6. Cutler, J., Schleihauf, E., Hatchette, T.F., Billard, B., Watson-Creed, G., Davidson, R., Li, Y., Bastien, N., and Sarwal, S. 2009. Investigation of the first cases of human-to-human infection with the new swine-origin influenza A (H1N1) virus in Canada. / CMAJ 181, 159-63. CrossRef
  7. Dawood, F.S., Iuliano, A.D., Reed, C., Meltzer, M.I., Shay, D.K., Cheng, P.Y., Bandaranayake, D., Breiman, R.F., Brooks, W.A., and Buchy, P. 2012. Estimated global mortality associated with the first 12 months of 2009 pandemic influenza A H1N1 virus circulation: a modelling study. / Lancet Infect. Dis. 12, 687-95. CrossRef
  8. Dhiman, N., Espy, M.J., Irish, C., Wright, P., Smith, T.F., and Pritt, B.S. 2010. Mutability in the matrix gene of novel influenza A H1N1 virus detected using a FRET probe-based real-time reverse transcriptase PCR assay. / J. Clin. Microbiol. 48, 677-79. CrossRef
  9. Dormitzer, P.R., Galli, G., Castellino, F., Golding, H., Khurana, S., Del Giudice, G., and Rappuoli, R. 2011. Influenza vaccine immunology. / Immunol. Rev. 239, 167-77. CrossRef
  10. Eisen, M.B., Sabesan, S., Skehel, J.J., and Wiley, D.C. 1997. Binding of the influenza A virus to cell-surface receptors: structures of five hemagglutinin-sialyloligosaccharide complexes determined by X-ray crystallography. / Virology 232, 19-1. CrossRef
  11. Gamblin, S., Haire, L., Russell, R., Stevens, D., Xiao, B., Ha, Y., Vasisht, N., Steinhauer, D., Daniels, R., and Elliot, A. 2004. The structure and receptor binding properties of the 1918 influenza hemagglutinin. / Science 303, 1838-842. CrossRef
  12. García-Ojeda, P.A., Hardy, S., Kozlowski, S., Stein, K.E., and Feavers, I.M. 2004. Surfa
• 刊物主题：Microbiology;
• 出版者：Springer Netherlands
• ISSN：1976-3794

文摘

Since the 2009 pandemic, monoclonal antibodies (mAbs) for rapid influenza diagnostic tests (RIDT) have been developed for specific diagnostics of pandemic viral infection. Most of the mAbs were poorly characterized because of urgency during the pandemic. Further characterization of the mAbs for RIDTs would be beneficial for understanding the immunological properties of the pandemic virus and utilizing the mAbs for other research purposes. In this study, it was confirmed that two mAbs (I38 and D383) in an RIDT for H1N1pdm09 diagnostics were able to detect H1N1pdm09 virus through enzyme-linked immunosorbent assay (ELISA) and immunofluorescence assay (IFA). Also, the two mAbs exhibited reactivity to hemagglutinins (HAs) of both the H1N1pdm09 and 1918 H1N1 viruses; therefore, the RIDT using the mAbs could detect HAs of H1N1pdm09 and also HAs of 1918 H1N1-like strains. In an extension to our previous study, the epitopes (Sa antigenic site and the interface area of F-and vestigial esterase subdomains on the HA1 domain of HA of H1N1pdm09) recognized by the mAbs were corroborated in depth by IFA with escape-mutants from the mAbs and mapping of the epitopes on the crystal structure of human H1N1 viral HAs. Collectively, these results imply that the mAbs for the RIDT may be suitable for use in studying the immunological properties of H1N1pdm09 viruses and that the Sa antigenic site and the interface area between F-and vestigial esterase subdomains on influenza viral HA recognized by the mAbs are immunologically conserved regions between H1N1pdm09 and 1918 H1N1.