Induction of apoptosis in cervical carcinoma HeLa cells by Petasites japonicus ethanol extracts

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• 作者：Yu-Jin Hwang (1) (2)
  Hae-Ri Wi (1)
  Haeng-Ran Kim (1)
  Kye Won Park (2)
  Kyung-A Hwang (1)
  
  1. Department of Agrofood Resources ; National Academy of Agricultural Science ; RDA ; Wanju ; Jeonbuk ; 565-850 ; Korea
  2. Department of Food Science and Biotechnology ; Sungkyunkwan University ; Suwon ; Gyeonggi ; 440-746 ; Korea
  
• 关键词：Petasites japonicus ; apoptosis ; intrinsic pathway ; caspase
• 刊名：Food Science and Biotechnology
• 出版年：2015
• 出版时间：April 2015
• 年：2015
• 卷：24
• 期：2
• 页码：665-672
• 全文大小：624 KB
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• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Nutrition
  Food Science
  
• 出版者：The Korean Society of Food Science and Technology in co-publication with Springer
• ISSN：2092-6456

文摘

Petasites japonicus is a traditional medicine in Korea for treating carcinoma, bronchitis, and asthma. However, the molecular mechanisms of anti-cancer activity are not well understood. The purpose of this study was to confirm the anti-cancer activity of P. japonicus and its mechanism. Apoptosis was examined using morphological observation of nuclear changes and flow cytometry. Activation of apoptosis-related markers was detected. P. japonicus extract significantly inhibited HeLa cell growth in vitro in a dose-dependent manner. Induction of apoptosis by P. japonicus was mediated by up-regulation of Bax and down-regulation of Bcl-2. P. japonicus also activated caspase-9, capspase-3, and PARP, indicating induction of the mitochondrial intrinsic apoptosis pathway. We also confirmed that administration of P. japonicus significantly reduced tumor weight in the xenografted mice. Thus, P. japonicus was effective for inhibiting tumor growth in vitro and in vivo by inducing apoptosis, suggesting its usefulness as a potential anti-cancer agent.