High-performance liquid chromatographic analysis for quantitation of marker compounds of Artemisia capillaris thunb.

详细信息    查看全文

• 作者：Kyung Min Park (1)
  Ying Li (1)
  Bora Kim (1)
  Haiyan Zhang (1)
  Kyong Hwangbo (1)
  Dong Gen Piao (1)
  Mei Juan Chi (1)
  Mi-Hee Woo (2)
  Jae Sue Choi (3)
  Je-Hyun Lee (4)
  Dong-Cheul Moon (5)
  Hyeun Wook Chang (1)
  Jae-Ryong Kim (6)
  Jong Keun Son (1)
  
• 关键词：Artemisia capillaris Thunb. ; Chlorogenic acid ; 3 ; 5 ; Di ; O ; caffeoylquinic acid ; 3 ; 4 ; Di ; O ; caffeoylquinic acid ; Scoparone ; HPLC
• 刊名：Archives of Pharmacal Research
• 出版年：2012
• 出版时间：December 2012
• 年：2012
• 卷：35
• 期：12
• 页码：2153-2162
• 全文大小：754KB
• 参考文献：1. Cha, J. D., Jeong, M. R., Jeong, S. I., Moon, S. E., Kim, J. Y., Kil, B. S., and Song, Y. H., Chemical composition and antimicrobial activity of the essential oils of / Artemisia scoparia and / A. capillaris. / Planta Med., 71, 186-90 (2005). CrossRef
  2. Cha, J. D., Moon, S. E., Kim, H. Y., Lee, J. C., and Lee, K. Y., The essential oil isolated from / Artemisia capillaris prevents LPS-induced production of NO and PGE2 by inhibiting MAPK-mediated pathways in RAW 264.7 macrophages. / Immunol. Invest., 38, 483-97 (2009a). CrossRef
  3. Cha, J. D., Moon, S. E., Kim, H. Y., Cha, I. H., and Lee, K. Y., Essential oil of / Artemisia capillaris induces apoptosis in KB cells via mitochondrial stress and caspase activation mediated by MAPK-stimulated signaling pathway. / J. Food Sci., 74, T75–T81 (2009b). CrossRef
  4. Choi, J. H., Kim, D. W., Yun, N., Choi, J. S., Islam, M. N., Kim, Y. S., and Lee, S. M., Protective effects of hyperoside against carbon tetrachloride-induced liver damage in mice. / J. Nat. Prod., 74, 1055-060 (2011). CrossRef
  5. Chu, C. Y., Tseng, T. H., Hwang, J. M., Chou, F. P., and Wang, C. J., Protective effects of capillarisin on tert-butylhydroperoxide- induced oxidative damage in rat primary hepatocytes. / Arch. Toxicol., 73, 263-68 (1999). CrossRef
  6. Guo, F. Q., Liang, Y. Z., Xu, C. J., Li, X. N., and Huang, L. F., Analyzing of the volatile chemical constituents in / Artemisia capillaris herba by GC-MS and correlative chemometric resolution methods. / J Pharm Biomed Anal., 35, 469-78 (2004). CrossRef
  7. Hong, J. H., Lee, J. W., Park, J. H., and Lee, I. S., Antioxidative and cytoprotective effects of / Artemisia capillaris fractions. / Biofactors, 31, 43-3 (2007). CrossRef
  8. Hong, J. H. and Lee, I. S., Effects of / Artemisia capillaris ethyl acetate fraction on oxidative stress and antioxidant enzyme in high-fat diet induced obese mice. / Chem. Biol. Interact., 179, 88-3 (2009a). CrossRef
  9. Hong, J. H. and Lee, I. S., Cytoprotective effect of / Artemisia capillaris fractions on oxidative stress-induced apoptosis in V79 cells. / Biofactors, 35, 380-88 (2009b). CrossRef
  10. Hu, Y. Q., Tan, R. X., Chu, M. Y., and Zhou, J., Apoptosis in human hepatoma cell line SMMC-7721 induced by watersoluble macromolecular components of / Artemisia capillaris Thunberg. / Jpn. J. Cancer Res., 91, 113-17 (2000). CrossRef
  11. Okuno, I., Uchida, K., Kadowaki, M., and Akahori, A., Choleretic effect of / Artemisia capillaris extract in rats. / Jpn. J. pharmacol., 31, 835-38 (1981). CrossRef
  12. Okuno, I., Uchida, K., Nakamura, M., and Sakurawi, K., Studies on choleretic constituents in / Artemisia capillaris Thunb. / Chem. Pharm. Bull., 36, 769-75 (1988). CrossRef
  13. Jang, S. I., Kim, Y. J., Lee, W. Y., Kwak, K. C., Baek, S. H., Kwak, G. B., Yun, Y. G., Kwon, T. O., Chung, H. T., and Chai, K. Y., Scoparone from / Artemisia capillaris inhibits the release of inflammatory mediators in RAW 264.7 cells upon stimulation cells by interferon-gamma plus LPS. / Arch. Pharm. Res., 28, 203-08 (2005). CrossRef
  14. Jang, S. I., Kim, Y. J., Kim, H. J., Lee, J. C., Kim, H. Y., Kim, Y. C., Yun, Y. G., Yu, H. H., and You, Y. O., Scoparone inhibits PMA-induced IL-8 and MCP-1 production through suppression of NF-κB activation in U937 cells. / Life Sci., 78, 2937-943 (2006). CrossRef
  15. Jung, H. A., Lee, H. J., Kim, Y. A., Park, K. E., Ahn, J. W., Lee, B. J., Moon, S. G., and Seo, Y. W., Antioxidant activity of / Artemisia capillaris Thunberg. / Food Sci. Biotechnol., 13, 328-31 (2004).
  16. Kim, Y. S., Bahn, K. N., Hah, C. K., Gang, H. I., and Ha, Y. L., Inhibition of 7,12-dimethylbenz[a]anthracene-induced mouse skin carcinogenesis by / Artemisia capillaris. / J. Food Sci., 73, T16–T20 (2008). CrossRef
  17. Kim, S. W., Kim, H. W., Woo, M. H., Lee, J. H., Choi, J. S., and Min, B. S., Quantitative determination of bioactive compounds in some / Artemisia capillaris by high-performance liquid chromatography. / Nat. Prod. Sci., 16, 233-38 (2010).
  18. Kimura, Y., Okuda, H., Okuda, T., Hatano, T., Agata, I., and Arichi, S., Studies on the activities of tannins and related compounds from medicinal plants and drugs. VII. Effects of extracts of leaves of / Artemisia species, and caffeic acid and chlorogenic acid on lipid metabolic injury in rats fed peroxidized oil. / Chem. Pharm. Bull., 33, 2028-034 (1985). CrossRef
  19. Kwon, O. S., Choi, J. S., Islam, M. N., Kim, Y. S., and Kim, H. P., Inhibition of 5-lipoxygenase and skin inflammation by the aerial parts of / Artemisia capillaris and its constituents. / Arch. Pharm. Res., 34, 1561-569 (2011). CrossRef
  20. Park, H. J., Nugroho, A., Lee, J., Kim, J. D., Kim, W. B., Lee, K. R., and Choi, J. S., HPLC analysis of caffeoylqunic acids in the extract of Cacalia firma and peroxynitrite scavenging effect. / Kor. J. Pharmacogn., 40, 365-69 (2009).
  21. Sheu, S. J., Chieh, C. L., and Weng, W. C., Capillary electrophoretic determination of the constituents of / Artemisiae Capillaris Herba. / J. Chromatogr. A, 911, 285-93 (2001). CrossRef
  22. Tien, Y. C., Liao, J. C., Chiu, C. S., Huang, T. H., Huang, C. Y., Chang, W. T., and Peng, W. H., Esculetin ameliorates carbon tetrachloride-mediated hepatic apoptosis in rats. / Int. J. Mol. Sci., 12, 4053-067 (2011). CrossRef
  23. Wang, H. L., Zou, H. F., Ni, J. Y., Kong, L., Gao, S., and Guo, B. C., Fractionation and analysis of / Artemisia capillaris Thunb. by affinity chromatography with human serum albumin as stationary phase. / J. Chromatogr. A, 870, 501-10 (2000). CrossRef
  24. Yang, C. C., Lee, M. R., Hsu, S. L., and Chang, C. M. J., Supercritical fluids extraction of capillarisin from / Artemisia capillaris and its inhibition of / in vitro growth of hepatoma cells. / J. Supercrit. Fluid, 42, 96-03 (2007). CrossRef
  
• 作者单位：Kyung Min Park (1)
  Ying Li (1)
  Bora Kim (1)
  Haiyan Zhang (1)
  Kyong Hwangbo (1)
  Dong Gen Piao (1)
  Mei Juan Chi (1)
  Mi-Hee Woo (2)
  Jae Sue Choi (3)
  Je-Hyun Lee (4)
  Dong-Cheul Moon (5)
  Hyeun Wook Chang (1)
  Jae-Ryong Kim (6)
  Jong Keun Son (1)
  
  1. College of Pharmacy, Yeungnam University, Gyeongsan, 712-749, Korea
  2. College of Pharmacy, Catholic University of Daegu, Gyeongsan, 712-702, Korea
  3. Faculty of Food Science and Biotechnology, Pukyoung National University, Busan, 608-737, Korea
  4. College of Oriental Medicine, Dongguk University, Gyeongju, 780-714, Korea
  5. College of Pharmacy, CBITRC, Chungbuk National University, Cheongju, 361-763, Korea
  6. Department of Biochemistry and Molecular Biology, Aging-associated Vascular Disease Research Center, College of Medicine, Yeungnam University, Daegu, 705-717, Korea
  
• ISSN：1976-3786

文摘

Two stable high-performance liquid chromatography (HPLC) methods were developed that could quantitatively analyze 10 major marker compounds of Artemisia capillaris Thunb and could also distinguish among ‘Injinho-and ‘Myeon-injin-and ‘Haninjin--A. capillaris collected in autumn, A. capillaris collected in spring and A. iwayomogi, which can be misused as ‘Injinho-in Korean herbal drug markets. The first HPLC method was a reversed-phase chromatography using a C18 column with an isocratic solvent system of phosphoric acid (0.05%) and acetonitrile at the flow rate of 1.0 mL/min, ultraviolet (UV) detection wavelength at 254 nm and column temperature at 40°C. Calibration and quantitation were made by using acetaminophen as an internal standard (I.S-A) and chlorogenic acid (1) was determined within 20 min. The second HPLC method was a reversed-phase chromatography using a C18 column with a gradient solvent system of phosphate buffer (0.015 M, pH 6) and acetonitrile at the flow rate of 1.0 mL/min, UV detection wavelength at 254 nm and column temperature at 40°C. Calibration and quantitation were made by using ethylparaben as an internal standard (I.S-B) and 3,5-di-O-caffeoylquinic acid (2), 3,4-di-O-caffeoylquinic acid (3), 4,5-di-O-caffeoylquinic acid (4), hyperoside (5), isoquercitrin (6), isorhamnetin 3-O-robinobioside (7), isorhamnetin-3-O-galactoside (8), isorhamnetin-3-O-glucoside (9) and scoparone (10) were determined within 60 min. Pattern recognition analysis of data from the 60 samples classified them clearly into three groups. These assay methods could be applied for QA/QC of A. capillaris and Artemisia iwayomogi.