Determination of glycoside hydrolase specificities during hydrolysis of plant cell walls using glycome profiling
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- 作者：Johnnie A. Walker ; Sivakumar Pattathil ; Lai F. Bergeman…
- 关键词：Glycoside hydrolase ; Xylanase ; Xyloglucanase ; Glycome profiling ; Nanostructure ; initiator mass spectrometry ; Enzyme specificity
- 刊名：Biotechnology for Biofuels
- 出版年：2017
- 出版时间：December 2017
- 年：2017
- 卷：10
- 期：1
- 全文大小：4736KB
- 刊物类别：Chemistry and Materials Science
- 刊物主题：Biotechnology; Plant Breeding/Biotechnology; Environmental Engineering/Biotechnology; Renewable and Green Energy; Renewable and Green Energy; Microbiology;
- 出版者：BioMed Central
- ISSN：1754-6834
- 卷排序：10
文摘
BackgroundGlycoside hydrolases (GHs) are enzymes that hydrolyze polysaccharides into simple sugars. To better understand the specificity of enzyme hydrolysis within the complex matrix of polysaccharides found in the plant cell wall, we studied the reactions of individual enzymes using glycome profiling, where a comprehensive collection of cell wall glycan-directed monoclonal antibodies are used to detect polysaccharide epitopes remaining in the walls after enzyme treatment and quantitative nanostructure initiator mass spectrometry (oxime-NIMS) to determine soluble sugar products of their reactions.