Investigation of the antibiotic complex produced by Lactococcus lactis subsp. lactis 194, Variant K

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• 作者：E. A. Ustyugova (1) ustyugova.katya@mail.ru
  G. B. Fedorova (2)
  G. S. Katrukha (2)
  L. G. Stoyanova (1) stoyanovamsu@mail.ru
• 关键词：environmental strain L. lactis subsp. lactis 194 – ; phase variation – ; antibiotic complex – ; isolation – ; physicochemical properties – ; identification
• 刊名：Microbiology
• 出版年：2011
• 出版时间：October 2011
• 年：2011
• 卷：80
• 期：5
• 页码：657-663
• 全文大小：209.5 KB
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• 作者单位：1. Biological Faculty, Moscow State University, Moscow, 119992 Russia2. Gause Institute of New Antibiotics, Russian Academy of Medical Sciences, ul. Bol鈥檚haya Pirogovskaya 11, Moscow, 119021 Russia
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Microbiology
  Medical Microbiology
  Russian Library of Science
  
• 出版者：MAIK Nauka/Interperiodica distributed exclusively by Springer Science+Business Media LLC.
• ISSN：1608-3237

文摘

Phase variation in the culture of the environmental strain Lactococcus lactis subsp. lactis 194 resulted in the formation of two types of colonies differing by 15% in antibiotic activity. The active variant 194-K produced an antibiotic complex with a broad spectrum of antibacterial and antifungal activity. Five components (194-A, B, C, D, and E) were isolated from the complex by solid-phase extraction and thin-layer chromatography. Components 194-A and 194-B were hydrophobic neutral compounds soluble in organic solvents. Component 194-A possessed fungicidal activity, whereas component 194-B exhibited only bactericidal activity. Physicochemical studies of the isolated components 194-A and 194-B revealed that they had no analogs in the Berdy database of biologically active substances (BNPD) and appeared to be novel antibiotics. Component 194-C was a hydrophilic polar compound inhibiting growth of gram-positive and gramnegative bacteria. Component 194-D belonged to peptide antibiotics; it inhibited growth of only gram-positive bacteria and was similar to nisin A in biological properties but differed in electrophoretic mobility and molecular mass.