DNA Folding Transition in Presence of Naphthylhydroxamic Acids as Revealed by Fluorescence Microscopic Single Molecule Observation Method

详细信息    查看全文

• 作者：Priyanka Singh ; Rama Pande
• 关键词：Fluorescence microscope ; Hydrodynamic radius ; Long ; axis length ; Naphthylhydroxamic acid
• 刊名：Journal of Fluorescence
• 出版年：2016
• 出版时间：January 2016
• 年：2016
• 卷：26
• 期：1
• 页码：67-73
• 全文大小：1,850 KB
• 参考文献：1.Rigler R, Mets U, Widengren J, Kask P (1993) Fluorescence correlation spectroscopy with high count rate and low background: analysis of translational diffusion. Eur Biophys J 22:169–175
  2.Takeshita F, Murai K, Iyama S, Ayukawa Y, Suetsugu T (1998) Uncontrolled diabetes hinders bone formation around titanium implants in rat tibiae. A light and fluorescence microscopy and image processing study. J Periodontol 69:314–320PubMed CrossRef
  3.Liu SC, Yi SJ, Mehta JR, Nichols PE, Ballas SK, Yacono PW, Golan DE, Palek J (1996) Red cell membrane remodeling in sickle cell anemia sequestration of membrane lipids and proteins in Heinz bodies. J Clin Invest 97:29–36PubMed PubMedCentral CrossRef
  4.Kassies R, Van Der Werf O, Lenferink A, Hunter CN, Olsen JD, Subramaniam V, Otto C (2005) Combined AFM and confocal fluorescence microscope for applications in bio-nanotechnology. J Microsc 217:109–116PubMed CrossRef
  5.Cherukuri P, Bachilo SM, Litovsky SH, Weisman BR (2004) Near-infrared fluorescence microscopy of single-walled carbon nanotubes in phagocytic cells. J Am Chem Soc 126:15638–15639PubMed CrossRef
  6.Robins B, Tysseland M (1983) The geology, geochemistry and origin of ultrabasic fenites associated with the Pollen Carbonatite. Chem Geol 40:65–95CrossRef
  7.Crelling JC (1983) Current uses of fluorescence microscopy in coal petrology. J Microsc 132:251–266CrossRef
  8.Khare D, Pande R (2012) Experimental and molecular docking study on DNA binding interaction of N-phenylbenzohydroxamic acid. Der Pharma Chemica 4:66–75
  9.Gupta VK, Tandon SG (1972) Preparation and properties of N-1-napthylhydroxamic acids. J Chem Eng Data 17:248–249CrossRef
  10.Yoshikawa Y, Yoshikawa K (1995) Diaminoalkanes with an odd number of carbon atoms induce compaction of a single double-stranded DNA chain. FEBS Lett 361:277–281PubMed CrossRef
  11.Yoshikawa K, Matsuzawa Y, Minagawa K, Doi M, Matsumoto M (1992) Biochem Biophys Res Commun 188:1274–1279PubMed CrossRef
  12.Sato YT, Hamada T, Kubo K, Yamada A, Kishida T, Mazda O, Yoshikawa K (2005) Folding transition into a loosely collapsed state in plasmid DNA as revealed by single-molecule observation. FEBS Lett 579:3095–3099PubMed CrossRef
  13.Oana H, Tsumoto K, Yoshikawa Y, Yoshikawa K (2002) Folding transition of large DNA completely inhibits the action of a restriction endonuclease as revealed by single-chain observation. FEBS Lett 530:143–146PubMed CrossRef
  14.Houseal TW, Bustamante C, Stump RF, Maestre MF (1989) Real-time imaging of single DNA molecules with fluorescence microscopy. Biophys J 56:507–516PubMed PubMedCentral CrossRef
  15.Flory EJ (1969) Statistical mechanics of chain molecules. Wiley, New York
  
• 作者单位：Priyanka Singh (1)
  Rama Pande (1)
  
  1. School of Studies in Chemistry, Pt. Ravishankar Shukla University, Raipur, 492010, Chhattisgarh, India
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Biomedicine
  Biomedicine
  Biophysics and Biomedical Physics
  Biotechnology
  Biochemistry
  Analytical Chemistry
  
• 出版者：Springer Netherlands
• ISSN：1573-4994

文摘

Fluorescence microscopy is a field of growing applications in various fields as it can be used to study immunofluorescence, fluorescent proteins techniques, live-cell imaging and many more. The present investigation is based on this single molecule observation technique to observe the effect of N-arylhydroxamic acids on λ plasmid DNA and linear Bacteriophage T7 DNA. The compounds under present investigation include N-1-naphthyl-o-methylbenzohydroxamic acid, N-1-naphthyl-p-methylbenzohydroxamic acid, N-1-naphthyl ethoxy benzo hydroxamic acid, N-1-naphthylphenylacetohydroxamic acid and N-1-naphthyl valero hydroxamic acid. The hydrodynamic radius, R _H of λ DNA and long-axis length, l of T7 DNA were determined from the direct observation of Brownian motion of the DNA molecules in the absence and presence of hydroxamic acids. Folding transition was observed for λ DNA as well as T7 DNA in the presence of naphthyl hydroxamic acids. N-1-naphthylvalerohydroxamic acid was found to be most effective. Keywords Fluorescence microscope Hydrodynamic radius Long-axis length Naphthylhydroxamic acid