Effect of Polyhydroxybutyrate (PHB) storage on l-arginine production in recombinant Corynebacterium crenatum using coenzyme regulation

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• 作者：Meijuan Xu ; Jingru Qin ; Zhiming Rao ; Hengyi You ; Xian Zhang…
• 关键词：l ; Arginine ; Poly ; β ; hydroxybutyrate (PHB) ; NAD kinase ; Corynebacterium crenatum SYPA 5
• 刊名：Microbial Cell Factories
• 出版年：2016
• 出版时间：December 2016
• 年：2016
• 卷：15
• 期：1
• 全文大小：2,080 KB
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• 作者单位：Meijuan Xu (1)
  Jingru Qin (1)
  Zhiming Rao (1)
  Hengyi You (1)
  Xian Zhang (1)
  Taowei Yang (1)
  Xiaoyuan Wang (1)
  Zhenghong Xu (2)
  
  1. The Key Laboratory of Industrial Biotechnology of Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, 214122, Jiangsu, People’s Republic of China
  2. Laboratory of Pharmaceutical Engineering, School of Medicine and Pharmaceutics, Jiangnan University, Wuxi, 214122, Jiangsu, People’s Republic of China
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Biotechnology
  Applied Microbiology
  Environmental Engineering/Biotechnology
  
• 出版者：BioMed Central
• ISSN：1475-2859

文摘

Background Corynebacterium crenatum SYPA 5 is the industrial strain for l-arginine production. Poly-β-hydroxybutyrate (PHB) is a kind of biopolymer stored as bacterial reserve materials for carbon and energy. The introduction of the PHB synthesis pathway into several strains can regulate the global metabolic pathway. In addition, both the pathways of PHB and l-arginine biosynthesis in the cells are NADPH-dependent. NAD kinase could upregulate the NADPH concentration in the bacteria. Thus, it is interesting to investigate how both PHB and NAD kinase affect the l-arginine biosynthesis in C. crenatum SYPA 5.