Enhanced production of poly-3-hydroxybutyrate by recombinant Escherichia coli containing NAD kinase and phbCAB operon
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- 作者：Shaoqin Zhang ; Lei Fang ; Zhengjun Li ; Yingying Guo…
- 关键词：PHB ; polyhydroxyalkanoates ; Tn5 transposon ; NAD kinase ; Escherichia coli
- 刊名：SCIENCE CHINA Chemistry
- 出版年：2016
- 出版时间：November 2016
- 年：2016
- 卷：59
- 期：11
- 页码：1390-1396
- 全文大小：815 KB
- 刊物类别：Chemistry and Materials Science
- 刊物主题：Chemistry
Chinese Library of Science
Chemistry - 出版者：Science China Press, co-published with Springer
- ISSN：1869-1870
- 卷排序：59
文摘
With the help of Tn5 transposon technique, gene yfjB encoding NAD kinase in Escherichia coli (E. coli) was inserted into chromosome of recombinant E. coli polyhydroxybutyrate (PHB) containing PHB synthesis operon integrated in the host genome. After successful transposition of an extra yfjB gene copy into genome, the selected recombinant named E. coli PHBTY4 showed stronger NAD kinase activity than that of E. coli PHB. Shake flask studies suggested that both cell dry weight and PHB accumulation were significantly increased in E. coli PHBTY4 compared with that of the control. E. coli PHBTY4 produced approximately 23 g/L PHB compared with its control which synthesized only 10 g/L PHB when grown under the same conditions in a 6 L fermentor after 32 h of cultivation. In addition, E. coli PHBTY4 maintained high genetic stability during the cultivation processes. These results revealed a practical method to construct genetically stable strains harboring extra NAD kinase gene to enhance NADP(H)-dependent bio-reactions.