Regulation of antimicrobial peptide synthesis in larvae of Calliphora vicina (Diptera, Calliphoridae): a dose-dependent effect of ecdysteroids

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• 作者：N. A. Gordya ; A. P. Nesin ; N. P. Simonenko…
• 关键词：insect immunity ; antimicrobial peptides ; 20 ; hydroxyecdysone ; ecdysteroids ; Calliphora
• 刊名：Journal of Evolutionary Biochemistry and Physiology
• 出版年：2016
• 出版时间：July 2016
• 年：2016
• 卷：52
• 期：4
• 页码：292-298
• 全文大小：168 KB
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Evolutionary Biology
  Biochemistry
  Animal Physiology
  Russian Library of Science
  
• 出版者：MAIK Nauka/Interperiodica distributed exclusively by Springer Science+Business Media LLC.
• ISSN：1608-3202
• 卷排序：52

文摘

Ecdysteroids are multifunctional hormones regulating virtually all morphogenetic events in insects. Their role in stress and immune response regulation is not well researched. Here we study the effect of 20-hydroxyecdysone on antimicrobial peptide synthesis in the larvae of Calliphora vicina. An inverse correlation was observed between the 20-hydroxyecdysone titer and antimicrobial peptide concentration in the hemolymph of naïve and bacteria challenged maggots. High and low doses of 20-hydroxyecdysone, injected simultaneously with bacterial cells, had an opposite effect on antimicrobial peptide synthesis in the diapausing larvae. Morphogenetically effective doses of 20-hydroxyecdysone demonstrated immunosuppressive activity. A low dose of 20-hydroxyecdysone, on the contrary, moderately stimulated antimicrobial peptide synthesis. The data suggest that ecdysteroids are directly involved in the regulation of the immune system activity and that the final effect is dose-dependant.