Separation of Amines as their 6-Methyl-2-phenyl-4-quinolinecarboxylic Acid N-Hydroxysuccinimide Ester Derivatives by High Performance Liquid Chromatography

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• 作者：Hong Wang ; Xin Liu ; Jin-Shu Li and Hua-Shan Zhang
• 关键词：Column liquid chromatography ; Fluorescent derivatizing reagent ; Aliphatic amines ; 6 ; Methyl ; 2 ; phenyl ; 4 ; quinolinecarboxylic acid N ; hydroxysuccinimide ester
• 刊名：Chromatographia
• 出版年：2005
• 出版时间：January 2005
• 年：2005
• 卷：61
• 期：1-2
• 页码：31-36
• 全文大小：333 KB
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Analytical Chemistry
  Organic Chemistry
  Pharmacy
  Biochemistry
  Plant Sciences
  Measurement Science and Instrumentation
  
• 出版者：Vieweg Verlag
• ISSN：1612-1112

文摘

6-Methyl-2-phenyl-4-quinolinecarboxylic acid N-hydroxysuccinimide ester (MPQC-OSu) has been developed for precolumn derivatization of aliphatic amines in HPLC. The great difference between the fluorescence quantum yield of MPQC-OSu derivative and that of MPQC-OSu hydrolysate, 6-methyl-2-phenyl-4-quinolinecarboxylic acid (MPQC) makes this reagent suitable for amine-labeling, followed by chromatographic separation. In pH8.5 borate buffer, MPQC-OSu reacted with amines at 60°C for 12 min to form highly fluorescent carboxamides and excess reagent hydrolysed to MPQC. The chromatographic behavior of amine derivatives with MPQC-OSu has been investigated using HPLC on C₁₈ and C₈ columns, respectively, with fluorescence detection at 340–402nm. A baseline separation of isopropanolamine, methylamine, ethylamine, n-propylamine, n-butylamine, n-amylamine and n-hexylamine was obtained in 25min using isocratic elution on a C₁₈ column using methanol-water (70:30, v/v) as eluent. Detection limits were in the range 0.13–1 nM.