Increased Vulnerability to Physical Stress by Inactivation of NdgR in Streptomyces coelicolor

详细信息    查看全文

• 作者：Bo-Rahm Lee (1)
  Da-Hye Yi (2)
  Eunjung Song (1)
  Shashi Kant Bhatia (2)
  Ju Hee Lee (2)
  Yun-Gon Kim (3)
  Sung-Hee Park (1)
  Yoo Kyung Lee (4)
  Byung-Gee Kim (1)
  Yung-Hun Yang (2) (5)
  
  1. School of Chemical and Biological Engineering ; Seoul National University ; Kwanak-Gu ; 151-742 ; Seoul ; Korea
  2. Department of Microbial Engineering ; College of Engineering ; Konkuk University ; 1 Hwayang-dong ; Gwangjin-gu ; Seoul ; 143-701 ; Korea
  3. Chemical Engineering ; Soongsil University ; 511 Sangdo-dong ; Seoul ; 156-743 ; Republic of Korea
  4. Division of Life Sciences ; Korea Polar Research Institute ; 12 Gaetbeol-ro ; Yeonsu-gu ; Incheon ; 406-840 ; Korea
  5. Institute for Ubiquitous Information Technology and Applications (CBRU) ; Konkuk University ; Seoul ; 143-701 ; South Korea
  
• 关键词：Streptomyces coelicolor ; ndgR ; Physical stress ; N ; acetylglucosamine ; Cell membrane
• 刊名：Applied Biochemistry and Biotechnology
• 出版年：2015
• 出版时间：April 2015
• 年：2015
• 卷：175
• 期：8
• 页码：3673-3682
• 全文大小：954 KB
• 参考文献：1. Beck, HC (2005) Branched-chain fatty acid biosynthesis in a branched-chain amino acid aminotransferase mutant of Staphylococcus carnosus. FEMS Microbiology Letters 243: pp. 37-44 CrossRef
  2. Bertram, R, Schlicht, M, Mahr, K, Nothaft, H, Saier, MH, Titgemeyer, F (2004) In silico and transcriptional analysis of carbohydrate uptake systems of Streptomyces coelicolor A3(2). Journal of Bacteriology 186: pp. 1362-1373 CrossRef
  3. Bibb, MJ (2005) Regulation of secondary metabolism in streptomycetes. Current Opinion in Microbiology 8: pp. 208-215 CrossRef
  4. Bystrykh, LV, Fernandez-Moreno, MA, Herrema, JK, Malpartida, F, Hopwood, DA, Dijkhuizen, L (1996) Production of actinorhodin-related 鈥渂lue pigments鈥?by Streptomyces coelicolor A3(2). Journal of Bacteriology 178: pp. 2238-2244
  5. Chater, KF (2001) Regulation of sporulation in Streptomyces coelicolor A3(2): a checkpoint multiplex?. Current Opinion in Microbiology 4: pp. 667-673 CrossRef
  6. Claessen, D, Jong, W, Dijkhuizen, L, Wosten, HA (2006) Regulation of Streptomyces development: reach for the sky!. Trends in Microbiology 14: pp. 313-319 CrossRef
  7. Druzhinina, IS, Schmoll, M, Seiboth, B, Kubicek, CP (2006) Global carbon utilization profiles of wild-type, mutant, and transformant strains of Hypocrea jecorina. Applied and Environmental Microbiology 72: pp. 2126-2133 CrossRef
  8. Fernandes, MF, Saxena, J, Dick, RP (2013) Comparison of whole-cell fatty acid (MIDI) or phospholipid fatty acid (PLFA) extractants as biomarkers to profile soil microbial communities. Microbial Ecology 66: pp. 145-157 CrossRef
  9. Fujii, T, Gramajo, HC, Takano, E, Bibb, MJ (1996) redD and actII-ORF4, pathway-specific regulatory genes for antibiotic production in Streptomyces coelicolor A3(2), are transcribed in vitro by an RNA polymerase holoenzyme containing sigma hrdD. Journal of Bacteriology 178: pp. 3402-3405
  10. Kieser, T, Bibb, MJ, Buttner, MJ, Chater, K, Hopwood, DA (2000) Practical streptomyces genetics. John Innes Centre, Norwich Research Park
  11. Kim, JN, Yi, JS, Lee, BR, Kim, EJ, Kim, MW, Song, Y, Cho, BK, Kim, BG (2012) A versatile PCR-based tandem epitope tagging system for Streptomyces coelicolor genome. Biochemical and Biophysical Research Communications 424: pp. 22-27 CrossRef
  12. Kim, SH, Lee, BR, Kim, JN, Kim, BG (2012) NdgR, a common transcriptional activator for methionine and leucine biosynthesis in Streptomyces coelicolor. Journal of Bacteriology 194: pp. 6837-6846 CrossRef
  13. Klein, W, Weber, MH, Marahiel, MA (1999) Cold shock response of Bacillus subtilis: isoleucine-dependent switch in the fatty acid branching pattern for membrane adaptation to low temperatures. Journal of Bacteriology 181: pp. 5341-5349
  14. Massey, LK, Sokatch, JR, Conrad, RS (1976) Branched-chain amino acid catabolism in bacteria. Bacteriological Reviews 40: pp. 42-54
  15. Mentzen, WI, Peng, J, Ransom, N, Nikolau, BJ, Wurtele, ES (2008) Articulation of three core metabolic processes in Arabidopsis: fatty acid biosynthesis, leucine catabolism and starch metabolism. BMC Plant Biology 8: pp. 76 CrossRef
  16. Park, SS, Yang, YH, Song, E, Kim, EJ, Kim, WS, Sohng, JK, Lee, HC, Liou, KK, Kim, BG (2009) Mass spectrometric screening of transcriptional regulators involved in antibiotic biosynthesis in Streptomyces coelicolor A3(2). Journal of Industrial Microbiology and Biotechnology 36: pp. 1073-1083 CrossRef
  17. Yang, YH, Song, E, Kim, EJ, Lee, K, Kim, WS, Park, SS, Hahn, JS, Kim, BG (2009) NdgR, an IclR-like regulator involved in amino-acid-dependent growth, quorum sensing, and antibiotic production in Streptomyces coelicolor. Applied Microbiology and Biotechnology 82: pp. 501-511 CrossRef
  18. Yang, YH, Song, E, Lee, BR, Kim, EJ, Park, SH, Kim, YG, Lee, CS, Kim, BG (2010) Rapid functional screening of Streptomyces coelicolor regulators by use of a pH indicator and application to the MarR-like regulator AbsC. Applied and Environmental Microbiology 76: pp. 3645-3656 CrossRef
  19. Yang, YH, Song, E, Willemse, J, Park, SH, Kim, WS, Kim, EJ, Lee, BR, Kim, JN, Wezel, GP, Kim, BG (2012) A novel function of Streptomyces integration host factor (sIHF) in the control of antibiotic production and sporulation in Streptomyces coelicolor. Antonie Van Leeuwenhoek 101: pp. 479-492 CrossRef
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Biotechnology
  Biochemistry
  
• 出版者：Humana Press Inc.
• ISSN：1559-0291

文摘

The antibiotic production and spore formation process in Streptomyces coelicolor need complex decision making processes by several regulatory units. These regulatory units are involved in both primary and secondary metabolism. As a result, most regulators have several functions, and those are worthwhile themes to study about different functions of a known regulator. In this study, a deletion mutant of ndgR, which encodes the nitrogen-dependent growth regulator, was examined by the cell viability test, TEM, and growth in N-acetylglucosamine/asparagine (GlcNAc/Asn) liquid medium. The results of the study show that NdgR is also involved in the structure of the cell membrane affecting survival under physical shocks. Deletion of ndgR leads to abnormal cell membrane resulting in the vulnerable cells to physical stress caused by shaking with beads in liquid culture condition. This empirical observation is the first meaningful explanation to why ndgR mutant could not grow well in a liquid minimal medium due to the defect of N-acetylglucosamine (GlcNAc) utilization and phospholipid synthesis.