Whole cell bioconversion of vitamin D3 to calcitriol using Pseudonocardia sp. KCTC 1029BP

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• 作者：Dae-Jung Kang ; Jong-Hyuk Im ; Jae-Hoon Kang…
• 关键词：Calcitriol ; Vitamin D3 ; Biocatalysis ; Calcifediol ; Pseudonocardia sp.
• 刊名：Bioprocess and Biosystems Engineering
• 出版年：2015
• 出版时间：July 2015
• 年：2015
• 卷：38
• 期：7
• 页码：1281-1290
• 全文大小：596 KB
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• 作者单位：Dae-Jung Kang (1) (2)
  Jong-Hyuk Im (2)
  Jae-Hoon Kang (2)
  Kyoung Heon Kim (1)
  
  1. Department of Biotechnology, Korea University Graduate School, Seoul, 136-713, Republic of Korea
  2. Research Laboratories, Ildong Pharmaceutical, Hwaseong, 445-170, Republic of Korea
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Biotechnology
  Industrial Chemistry and Chemical Engineering
  Industrial and Production Engineering
  Waste Management and Waste Technology
  Waste Water Technology, Water Pollution Control, Water Management and Aquatic Pollution
  Food Science
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1615-7605

文摘

Calcitriol is an important drug used for treating osteoporosis, which can be produced from vitamin D3. The current method of producing calcitriol from vitamin D3 during cultivation of microbial cells results in low yields of calcitriol and high purification costs. Therefore, in this study, the steps of cell cultivation and bioconversion of vitamin D3 to calcitriol were separated. Cells of Pseudonocardia sp. KCTC 1029BP were utilized as a whole cell catalyst to produce a high level and yield of calcitriol from vitamin D3. In addition, the effects of bioconversion buffers, cyclodextrins, and metal salts on the production of calcitriol were comparatively examined and selected for incorporation in the bioconversion medium, and their compositions were statistically optimized. The optimal bioconversion medium was determined as consisting of 15?mM Trizma base, 25?mM sodium succinate, 2?mM MgSO4, 0.08?% β-cyclodextrin, 0.1?% NaCl, 0.2?% K2HPO4, and 0.03?% MnCl2. Using this optimal bioconversion medium, 61.87?mg/L of calcitriol, corresponding to a 30.94?% mass yield from vitamin D3, was produced in a 75-L fermentor after 9?days. This calcitriol yield was 3.6 times higher than that obtained using a bioconversion medium lacking β-cyclodextrin, NaCl, K2HPO4, and MnCl2. In conclusion, utilizing whole cells of Pseudonocardia sp. KCTC 1029BP together with the optimal bioconversion medium markedly enhanced the production of calcitriol from vitamin D3.