Phylogenetic relationship between symbionts of tubeworm Lamellibrachia satsuma and the sediment microbial community in Kagoshima Bay

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• 作者：Ajit Kumar Patra ; Hyun Hee Cho ; Yong Min Kwon ; Kae Kyoung Kwon…
• 关键词：Key wordsLamellibrachia satsuma ; symbionts ; tubeworm ; microbial diversity in sediment ; Kagoshima Bay
• 刊名：Ocean Science Journal
• 出版年：2016
• 出版时间：September 2016
• 年：2016
• 卷：51
• 期：3
• 页码：317-332
• 全文大小：1,840 KB
• 刊物类别：Earth and Environmental Science
• 刊物主题：Geosciences
  Oceanography
  Waste Water Technology, Water Pollution Control, Water Management and Aquatic Pollution
  
• 出版者：Korean Ocean Research and Development Institute (KORDI) and the Korean Society of Oceanography, co-p
• ISSN：2005-7172
• 卷排序：51

文摘

Vestimentiferan tubeworms acquire their symbionts through horizontal transmission from the surrounding environment. In the present study, we constructed a 16S rRNA gene clone library to investigate the phylogenetic relationship between diverse microbes in the sediment and symbiotic bacteria in the trophosome of the tubeworm, Lamellibrachia satsuma, from Kagoshima Bay, Japan. Two symbiotic bacterial phylotypes belonging to the classes γ- and ε-Proteobacteria were found from this tubeworm trophosome. They were very closely related to the symbionts of several other marine invertebrates. The most predominant bacteria in the sediment were ε-Proteobacteria. A broad diversity of bacteria belonged to non-proteobacterial phyla such as Planctomycetes, Acidobacteria, and Chloroflexi was observed. The presence of sulfur oxidizers (i.e., ε-Proteobacteria and γ-Proteobacteria) and sulfur reducers (i.e., δ-Proteobacteria) may play a significant role in the sulfur cycle in these habitats and provide multiple sources of nutrition to the cold-seep communities. Closely related clones of ε-Proteobacteria symbiont in the species level and of γ-Proteobacteria symbiont in the genus level were found in the surrounding sediment. The similarity of symbiont clones of L. satsuma with other symbionts and free-living bacteria suggests the possibility of opportunistic symbiosis in ε-Proteobacteria and the co-evolution of γ-Proteobacteria having occurred after symbiosis with the tubeworms.