Sensitive change of iso-branched fatty acid (iso-15:0) in Bacillus pumilus PAMC 23174 in response to environmental changes

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• 作者：Da-Hye Yi ; Ganesan Sathiyanarayanan ; Hyung Min Seo…
• 关键词：Polar bacteria ; Bacillus pumilus ; Climate change ; Adaptation ; FAME ; GC–MS
• 刊名：Bioprocess and Biosystems Engineering
• 出版年：2016
• 出版时间：January 2016
• 年：2016
• 卷：39
• 期：1
• 页码：159-167
• 全文大小：1,294 KB
• 参考文献：1.Kempf B, Bremer E (1998) Uptake and synthesis of compatible solutes as microbial stress responses to high-osmolality environments. Arch Microbiol 170(5):319–330CrossRef
  2.Zhu K, Ding X, Julotok M, Wilkinson BJ (2005) Exogenous isoleucine and fatty acid shortening ensure the high content of anteiso-C-15: 0 fatty acid required for low-temperature growth of Listeria monocytogenes. Appl Environ Microbiol 71(12):8002–8007CrossRef
  3.Giotis ES, McDowell DA, Blair IS, Wilkinson BJ (2007) Role of branched-chain fatty acids in pH stress tolerance in Listeria monocytogenes. Appl Environ Microbiol 73(3):997–1001CrossRef
  4.Pepi M, Heipieper HJ, Fischer J, Ruta M, Volterrani M, Focardi SE (2008) Membrane fatty acids adaptive profile in the simultaneous presence of arsenic and toluene in Bacillus sp ORAs2 and Pseudomonas sp ORAs5 strains. Extremophiles 12(3):343–349CrossRef
  5.Kaneda T (1967) Fatty acids in the genus Bacillus. I. Iso- and anteiso-fatty acids as characteristic constituents of lipids in 10 species. J Bacteriol 93(3):894–903
  6.Kaneda T (1963) Valine as a source of the branched short chain precursor in the biosynthesis if iso-C14, iso-C15, iso-C16 and iso-C17 fatty acids by Bacillus subtitis. Biochem Biophys Res Commun 10(3):283–287CrossRef
  7.Murata T (1978) Analysis of fatty acid methyl esters by a gas–liquid chromatography–chemical ionization mass spectrometry computer system. J Lipid Res 19(2):166–171
  8.Takemoto Y, Suzuki Y, Horibe R, Shimozawa N, Wanders RJ, Kondo N (2003) Gas chromatography/mass spectrometry analysis of very long chain fatty acids, docosahexaenoic acid, phytanic acid and plasmalogen for the screening of peroxisomal disorders. Brain Dev 25(7):481–487CrossRef
  9.Delille D, Perret E (1989) Influence of temperature on the growth potential of Southern polar marine bacteria. Microb Ecol 18(2):117–123CrossRef
  10.Knoblauch C, Jorgensen BB (1999) Effect of temperature on sulphate reduction, growth rate and growth yield in five psychrophilic sulphate-reducing bacteria from Arctic sediments. Environ Microbiol 1(5):457–467CrossRef
  11.Kaneda T (1963) Biosnythesis of branched chain fatty acids. I. Isolation and identification of fatty acids from Bacillus subtilis (ATCC 7059). J Biol Chem 238(4):1222–1228
  12.Kaneda T (1966) Biosynthesis of branched-chain fatty acids. IV. Factors affecting relative abundance of fatty acids produced by Bacillus subtilis. Can J Microbiol 12(3):501–514CrossRef
  13.Kaneda T (1991) Iso- and anteiso-fatty acids in bacteria: biosynthesis, function, and taxonomic significance. Microbiol Rev 55(2):288–302
  14.Graumann PL, Marahiel MA (1999) Cold shock response in Bacillus subtilis. J Mol Microbiol Biotechnol 1(2):203–209
  15.Klein W, Weber MH, Marahiel MA (1999) Cold shock response of Bacillus subtilis: isoleucine-dependent switch in the fatty acid branching pattern for membrane adaptation to low temperatures. J Bacteriol 181(17):5341–5349
  16.Zhu K, Ding X, Julotok M, Wilkinson BJ (2005) Exogenous isoleucine and fatty acid shortening ensure the high content of anteiso-C15: 0 fatty acid required for low-temperature growth of Listeria monocytogenes. Appl Environ Microbiol 71(12):8002–8007CrossRef
  17.Bayles DO, Annous BA, Wilkinson BJ (1996) Cold stress proteins induced in Listeria monocytogenes in response to temperature downshock and growth at low temperatures. Appl Environ Microbiol 62(3):1116–1119
  18.Nickerson KW, Bulla LA Jr, Mounts TL (1975) Lipid metabolism during bacterial growth, sporulation, and germination: differential synthesis of individual branched- and normal-chain fatty acids during spore germination and outgrowth of Bacillus thuringiensis. J Bacteriol 124(3):1256–1262
  19.Naik DN, Kaneda T (1974) Biosynthesis of branched long-chain fatty acids by species of Bacillus: relative activity of three alpha-keto acid substrates and factors affecting chain length. Can J Microbiol 20(12):1701–1708CrossRef
  20.Belitsky BR (2015) Role of branched-chain amino acid transport in Bacillus subtilis CodY activity. J Bacteriol 197(8):1330–1338CrossRef
  21.Goldstein BJ, Zahler SA (1976) Uptake of branched-chain alpha-keto acids in Bacillus subtilis. J Bacteriol 127:667–670
  22.Kaneda T (1977) Fatty acids of the genus Bacillus: an example of branched-chain preference. Bacteriol Rev 41(2):391
  23.Wang GF, Kuriki T, Roy KL, Kaneda T (1993) The primary structure of branched-chain alpha-oxo acid dehydrogenase from Bacillus subtilis and its similarity to other alpha-oxo acid dehydrogenases. Eur J Biochem 213(3):1091–1099CrossRef
  24.Perham RN, Lowe PN (1988) Isolation and properties of the branched-chain 2-keto acid and pyruvate dehydrogenase multienzyme complex from Bacillus subtilis. Methods Enzymol 166:330–342CrossRef
  25.Poralla K (1971) The induction of a dehydrogenase activity for branched chain amino acids in Bacillus subtilis. Arch Mikrobiol 77(4):339–343CrossRef
  26.Conner RM, Hansen PA (1967) Effects of valine, leucine, and isoleucine on the growth of Bacillus thuringiensis and related bacteria. J Invertebr Pathol 9(1):12–18CrossRef
  
• 作者单位：Da-Hye Yi (1)
  Ganesan Sathiyanarayanan (1)
  Hyung Min Seo (1)
  Jung-Ho Kim (1)
  Shashi Kant Bhatia (1)
  Yun-Gon Kim (2)
  Sung-Hee Park (3)
  Ji-Young Jung (4)
  Yoo Kyung Lee (4)
  Yung-Hun Yang (1) (5)
  
  1. Department of Biological Engineering, College of Engineering, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul, 143-701, South Korea
  2. Chemical Engineering, Soongsil University, 511 Sangdo-dong, Seoul, 156-743, South Korea
  3. Food Ingredients Center, Foods R and D, CheilJedang, Guro-dong, Guro-Gu, Seoul, 152-051, South Korea
  4. Division of Life Sciences, Korea Polar Research Institute, 12 Gaetbeol-ro, Yeonsu-gu, Incheon, 406-840, South Korea
  5. Microbial Carbohydrate Resource Bank, Konkuk University, Seoul, 143-701, South Korea
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Biotechnology
  Industrial Chemistry and Chemical Engineering
  Industrial and Production Engineering
  Waste Management and Waste Technology
  Waste Water Technology, Water Pollution Control, Water Management and Aquatic Pollution
  Food Science
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1615-7605

文摘

In this study, the environmental adaptive metabolic processes were investigated using a psychrotrophic polar bacterium Bacillus pumilus PAMC 23174 in response to various temperatures and nutrients, especially in regard to the synthesis of fatty acids. Fatty acid methyl ester analysis was performed using gas chromatography–mass spectrometry and we found that a sensitive changes in iso-branched fatty acid (iso-15:0) synthesis occurred when adjusting the nutritional ratio of branched chain fatty acids (anteiso/iso) with different temperatures, resulting in a change in the balance of anteiso- and iso-form fatty acids. We also observed that this Arctic bacterium preferred amino acid leucine for the synthesis of fatty acids. The increased and decreased synthesis of iso-form fatty acids in response to different temperatures and leucine preference, changes the fatty acid ratio in bacteria, which further affects the membrane fluidity and it is also directly correlated with survival of bacteria in an extreme environment. Hence, this study suggests that B. pumilus PAMC 23174 is a potential model organism for the analysis of the unique ecological adaptations of polar bacteria in changing and the extreme environments.